Fibrinography: A Multiwavelength Light-Scattering Assay of Fibrin Structure.


Journal

HemaSphere
ISSN: 2572-9241
Titre abrégé: Hemasphere
Pays: United States
ID NLM: 101740619

Informations de publication

Date de publication:
Feb 2019
Historique:
received: 02 10 2018
accepted: 20 11 2018
entrez: 15 11 2019
pubmed: 15 11 2019
medline: 15 11 2019
Statut: epublish

Résumé

We have previously developed a fibrin structural assay dedicated to purified fibrinogen-thrombin system. Here, we extend the pertinence of this test, called Fibrinography, to tissue factor-triggered plasma coagulation. We show that Fibrinography determines quantitatively the structure of fibrin fibers in plasma with an excellent reproducibility. We compare this assay with the commonly used single wavelength turbidity method, showing that the latter is not a proper structural assay, but determines essentially the fibrinogen content in plasma. In addition, we also show, in model plasmas, that Fibrinography is able to discriminate normal and hypocoagulant plasmas, and even between hypercoagulant plasmas. Therefore, Fibrinography, by measuring the final step of the coagulation cascade, may be used to evaluate patients' plasma in hypo- or hypercoagulant diseases.

Identifiants

pubmed: 31723805
doi: 10.1097/HS9.0000000000000166
pii: HemaSphere-2018-0179
pmc: PMC6745935
doi:

Types de publication

Journal Article

Langues

eng

Pagination

e166

Informations de copyright

Copyright © 2019 the Author(s). Published by Wolters Kluwer Health, Inc. on behalf of the European Hematology Association.

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Auteurs

Carhel Dassi (C)

Laboratoire TIMC-IMAG/TheREx, Université Grenoble Alpes, CNRS UMR 5525, Département d'Hématologie, Institut de Biologie et de Pathologie, Centre Hospitalier Universitaire Grenoble-Alpes, Grenoble, France.

Landry Seyve (L)

Laboratoire TIMC-IMAG/TheREx, Université Grenoble Alpes, CNRS UMR 5525, Département d'Hématologie, Institut de Biologie et de Pathologie, Centre Hospitalier Universitaire Grenoble-Alpes, Grenoble, France.

Xabel García (X)

Laboratoire Rhéologie et Procédés, Université Grenoble Alpes, CNRS UMR 5520, Grenoble, France.

Emmanuelle Bigo (E)

Laboratoire TIMC-IMAG/TheREx, Université Grenoble Alpes, CNRS UMR 5525, Département d'Hématologie, Institut de Biologie et de Pathologie, Centre Hospitalier Universitaire Grenoble-Alpes, Grenoble, France.

Raphaël Marlu (R)

Laboratoire TIMC-IMAG/TheREx, Université Grenoble Alpes, CNRS UMR 5525, Département d'Hématologie, Institut de Biologie et de Pathologie, Centre Hospitalier Universitaire Grenoble-Alpes, Grenoble, France.

François Caton (F)

Laboratoire Rhéologie et Procédés, Université Grenoble Alpes, CNRS UMR 5520, Grenoble, France.

Benoît Polack (B)

Laboratoire TIMC-IMAG/TheREx, Université Grenoble Alpes, CNRS UMR 5525, Département d'Hématologie, Institut de Biologie et de Pathologie, Centre Hospitalier Universitaire Grenoble-Alpes, Grenoble, France.

Classifications MeSH