A Listeria monocytogenes Bacteriocin Can Target the Commensal Prevotella copri and Modulate Intestinal Infection.
Animals
Anti-Bacterial Agents
/ pharmacology
Bacteriocins
/ pharmacology
Female
Gastrointestinal Microbiome
/ drug effects
Germ-Free Life
Humans
Inflammation
/ prevention & control
Listeria monocytogenes
/ metabolism
Listeriosis
/ microbiology
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Prevotella
/ drug effects
Bacteriocin
Listeria monocytogenes
Lmo2776
M-SHIME
Prevotella copri
infection
microbiota
Journal
Cell host & microbe
ISSN: 1934-6069
Titre abrégé: Cell Host Microbe
Pays: United States
ID NLM: 101302316
Informations de publication
Date de publication:
13 11 2019
13 11 2019
Historique:
received:
30
06
2019
revised:
05
09
2019
accepted:
23
10
2019
entrez:
15
11
2019
pubmed:
15
11
2019
medline:
5
3
2020
Statut:
ppublish
Résumé
Understanding the role of the microbiota components in either preventing or favoring enteric infections is critical. Here, we report the discovery of a Listeria bacteriocin, Lmo2776, which limits Listeria intestinal colonization. Oral infection of conventional mice with a Δlmo2776 mutant leads to a thinner intestinal mucus layer and higher Listeria loads both in the intestinal content and deeper tissues compared to WT Listeria. This latter difference is microbiota dependent, as it is not observed in germ-free mice. Strikingly, it is phenocopied by pre-colonization of germ-free mice before Listeria infection with Prevotella copri, an abundant gut-commensal bacteria, but not with the other commensals tested. We further show that Lmo2776 targets P. copri and reduces its abundance. Together, these data unveil a role for P.copri in exacerbating intestinal infection, highlighting that pathogens such as Listeria may selectively deplete microbiota bacterial species to avoid excessive inflammation.
Identifiants
pubmed: 31726031
pii: S1931-3128(19)30540-2
doi: 10.1016/j.chom.2019.10.016
pmc: PMC6854461
pii:
doi:
Substances chimiques
Anti-Bacterial Agents
0
Bacteriocins
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
691-701.e5Subventions
Organisme : NCATS NIH HHS
ID : TL1 TR001447
Pays : United States
Organisme : Howard Hughes Medical Institute
Pays : United States
Commentaires et corrections
Type : CommentIn
Informations de copyright
Copyright © 2019 The Authors. Published by Elsevier Inc. All rights reserved.
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