CAMDI interacts with the human memory-associated protein KIBRA and regulates AMPAR cell surface expression and cognition.


Journal

PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081

Informations de publication

Date de publication:
2019
Historique:
received: 08 05 2019
accepted: 25 10 2019
entrez: 16 11 2019
pubmed: 16 11 2019
medline: 24 3 2020
Statut: epublish

Résumé

Little is known about the molecular mechanisms of cognitive deficits in psychiatric disorders. CAMDI is a psychiatric disorder-related factor, the deficiency of which in mice results in delayed neuronal migration and psychiatrically abnormal behaviors. Here, we found that CAMDI-deficient mice exhibited impaired recognition memory and spatial reference memory. Knockdown of CAMDI in hippocampal neurons increased the amount of internalized alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate receptor (AMPAR) and attenuated the chemical long-term potentiation (LTP)-dependent cell surface expression of AMPAR. KIBRA was identified as a novel CAMDI-binding protein that retains AMPAR in the cytosol after internalization. KIBRA inhibited CAMDI-dependent Rab11 activation, thereby attenuating AMPAR cell surface expression. These results suggest that CAMDI regulates AMPAR cell surface expression during LTP. CAMDI dysfunction may partly explain the mechanism underlying cognitive deficits in psychiatric diseases.

Identifiants

pubmed: 31730661
doi: 10.1371/journal.pone.0224967
pii: PONE-D-19-13012
pmc: PMC6857912
doi:

Substances chimiques

CCDC141 protein, mouse 0
Intracellular Signaling Peptides and Proteins 0
Nerve Tissue Proteins 0
Phosphoproteins 0
Receptors, AMPA 0
Wwc1 protein, mouse 0
rab11 protein EC 3.6.1.-
rab GTP-Binding Proteins EC 3.6.5.2

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e0224967

Déclaration de conflit d'intérêts

The authors have declared that no competing interests exist.

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Auteurs

Toshifumi Fukuda (T)

Laboratory of Molecular Biochemistry, School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo, Japan.

Shun Nagashima (S)

Laboratory of Molecular Biochemistry, School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo, Japan.

Ryoko Inatome (R)

Laboratory of Molecular Biochemistry, School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo, Japan.

Shigeru Yanagi (S)

Laboratory of Molecular Biochemistry, School of Life Sciences, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo, Japan.

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Classifications MeSH