An ultrasensitive microchip electrophoresis chemiluminescence assay platform for detection of trace biomolecules.
Chemiluminescence detection
Gamma interferon
Microchip electrophoresis
Signal amplification
Ultrasensitive assay
Journal
Journal of chromatography. A
ISSN: 1873-3778
Titre abrégé: J Chromatogr A
Pays: Netherlands
ID NLM: 9318488
Informations de publication
Date de publication:
22 Feb 2020
22 Feb 2020
Historique:
received:
15
09
2019
revised:
05
11
2019
accepted:
07
11
2019
pubmed:
17
11
2019
medline:
21
4
2020
entrez:
17
11
2019
Statut:
ppublish
Résumé
An ultrasensitive microchip electrophoresis chemiluminescence (MCE-CL) assay platform based on separation assisted cascade signal amplification was developed for detection of trace biomolecules. In this work, the aptamer was used as a target probe to bind target molecule and triggering cascade signal amplification reaction. The horseradish peroxide labeled DNA (HRP-DNA) was used as signal probe, utilizing nucleic acid hybridization and exonuclease cutting technology realized ultrasensitive detection of biomolecules on the MCE-CL assay platform. Taking gamma interferon (IFN-γ) as a model analyte, the linear range for IFN-γ detection is 8.0 × 10
Identifiants
pubmed: 31732154
pii: S0021-9673(19)31122-7
doi: 10.1016/j.chroma.2019.460693
pii:
doi:
Substances chimiques
Aptamers, Nucleotide
0
DNA Probes
0
IFNG protein, human
0
Peroxides
0
Interferon-gamma
82115-62-6
DNA
9007-49-2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
460693Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The author(s) declare that they have no competing interest.