Age-related decrease in muscle satellite cells is accompanied with diminished expression of early growth response 3 in mice.
Age Factors
Aging
/ genetics
Animals
Cells, Cultured
Early Growth Response Protein 3
/ genetics
Gene Expression Regulation
/ genetics
Male
Mice
Mice, Inbred C57BL
Muscle Cells
/ metabolism
Muscle Development
/ physiology
Muscle, Skeletal
/ physiology
PAX7 Transcription Factor
/ genetics
Regeneration
Satellite Cells, Skeletal Muscle
/ metabolism
Wound Healing
FACS
Myogenesis
Regeneration
Sarcopenia
Stem cells
Journal
Molecular biology reports
ISSN: 1573-4978
Titre abrégé: Mol Biol Rep
Pays: Netherlands
ID NLM: 0403234
Informations de publication
Date de publication:
Feb 2020
Feb 2020
Historique:
received:
31
07
2019
accepted:
09
11
2019
pubmed:
18
11
2019
medline:
24
6
2020
entrez:
18
11
2019
Statut:
ppublish
Résumé
Skeletal muscle regeneration is mostly dependent on muscle satellite cells. Proper muscle regeneration requires enough number of satellite cells. Recent studies have suggested that the number of satellite cells in skeletal muscle declines as we age, leading to the impairment of muscle regeneration in older population. Our earlier study demonstrated that zinc finger transcription factor early growth response 3 (Egr3) plays an important role for maintaining the number of myoblasts, suggesting that age-related decrease in muscle satellite cell should be associated with the expression levels of Egr3. The aim of this study was to investigate whether aging would alter the Egr3 expression in satellite cells. A couple groups of male C57BL/6J mice were examined in this study: young (3 Mo) and old (17 Mo). Immunohistochemical staining showed that the satellite cell number decreased in normal and injured muscles of old mice. In fluorescence-activated cell sorting-isolated muscle satellite cells from normal and injured muscles, the mRNA expression of Egr3 was significantly decreased with age regardless of injury. In harmony with these results, Pax7 mRNA levels also decreased in the satellite cells from old mice. Alternatively, inhibition of Egr3 expression by shRNA decreased Pax7 protein expression in cultured myoblasts. These results suggest that Egr3 is associated with the age-related decline of muscle satellite cells in older population. Also, Egr3 might be implicated in the regulation of Pax7. Therefore, the loss of Egr3 expression may elucidate attenuated MSCs function and muscle regeneration in older age.
Identifiants
pubmed: 31734897
doi: 10.1007/s11033-019-05189-5
pii: 10.1007/s11033-019-05189-5
doi:
Substances chimiques
Egr3 protein, mouse
0
PAX7 Transcription Factor
0
Pax7 protein, mouse
0
Early Growth Response Protein 3
144516-98-3
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
977-986Subventions
Organisme : Japan Society for the Promotion of Science
ID : 18K10835
Organisme : Japan Society for the Promotion of Science
ID : 15K01633
Organisme : Japan Society for the Promotion of Science
ID : 15K01632
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