Antileishmanial effect of rapamycin as an alternative approach to control Leishmania tropica infection.
Amphotericin B
/ pharmacology
Animals
Antiprotozoal Agents
/ pharmacology
Cell Line, Tumor
Cytokines
/ analysis
Female
Humans
Inhibitory Concentration 50
Leishmania tropica
/ drug effects
Leishmaniasis, Cutaneous
/ drug therapy
Lymph Nodes
/ parasitology
Meglumine Antimoniate
/ pharmacology
Mice
Mice, Inbred BALB C
Microscopy, Electron, Scanning
Microscopy, Electron, Transmission
Parasite Load
Random Allocation
Sirolimus
/ pharmacology
TOR Serine-Threonine Kinases
/ drug effects
BALB/c mouse model
Combination therapy
Cutaneous leishmaniosis
L. tropica
Rapamycin
mTOR
Journal
Veterinary parasitology
ISSN: 1873-2550
Titre abrégé: Vet Parasitol
Pays: Netherlands
ID NLM: 7602745
Informations de publication
Date de publication:
Dec 2019
Dec 2019
Historique:
received:
27
04
2019
revised:
05
11
2019
accepted:
09
11
2019
pubmed:
19
11
2019
medline:
2
1
2020
entrez:
19
11
2019
Statut:
ppublish
Résumé
Cutaneous leishmaniosis (CL) is a parasitic disease in animals and human with no satisfactory treatments and vaccination. Rapamycin is a potent inhibitor of mammalian target of rapamycin (mTOR) with various applications. Here, the effect of rapamycin alone or in combination with two other drugs, namely amphotericin B (AmB) and glucantime, was investigated against Leishmania tropica infection. In vitro viability and electron microscopy evaluation of the parasites showed detrimental changes in their appearance and viability. Treatment with clinically relevant dose of rapamycin (10.2 μg/dose) is able to control the parasite load in BALB/c mice infected with L. tropica. Furthermore, the cytokine profiles showed significant polarization towards Th1 immune response. Surprisingly, combination therapy with either AmB or glucantime was not efficient. Rapamycin is showed an effective alternative therapy against leishmaniosis caused by L. tropica.
Identifiants
pubmed: 31739256
pii: S0304-4017(19)30257-2
doi: 10.1016/j.vetpar.2019.108976
pii:
doi:
Substances chimiques
Antiprotozoal Agents
0
Cytokines
0
Meglumine Antimoniate
75G4TW236W
Amphotericin B
7XU7A7DROE
TOR Serine-Threonine Kinases
EC 2.7.11.1
Sirolimus
W36ZG6FT64
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
108976Commentaires et corrections
Type : ErratumIn
Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.