Modulation of autophagy by RTN-1C: role in autophagosome biogenesis.
Journal
Cell death & disease
ISSN: 2041-4889
Titre abrégé: Cell Death Dis
Pays: England
ID NLM: 101524092
Informations de publication
Date de publication:
18 11 2019
18 11 2019
Historique:
received:
08
03
2019
accepted:
14
10
2019
revised:
04
10
2019
entrez:
20
11
2019
pubmed:
20
11
2019
medline:
25
9
2020
Statut:
epublish
Résumé
The endoplasmic reticulum (ER) is a key organelle fundamental for the maintenance of cellular homeostasis and to determine the cell's fate under stress conditions. Among the known proteins that regulate ER structure and function there is Reticulon-1C (RTN-1C), a member of the reticulon family localized primarily on the ER membrane. We previously demonstrated that RTN-1C expression affects ER function and stress condition. ER is an essential site for the regulation of apoptotic pathways and it has also been recently recognized as an important component of autophagic signaling. Based on these evidences, we have investigated the impact of RTN-1C modulation on autophagy induction. Interestingly we found that reticulon overexpression is able to activate autophagic machinery and its silencing results in a significative inhibition of both basal and induced autophagic response. Using different experimental approaches we demonstrated that RTN-1C colocalizes with ATG16L and LC3II on the autophagosomes. Considering the key role of reticulon proteins in the control of ER membrane shaping and homeostasis, our data suggest the participation of RTN-1C in the autophagic vesicle biogenesis at the level of the ER compartment. Our data indicate a new mechanism by which this structural ER protein modulates cellular stress, that is at the basis of different autophagy-related pathologies.
Identifiants
pubmed: 31740665
doi: 10.1038/s41419-019-2099-7
pii: 10.1038/s41419-019-2099-7
pmc: PMC6861279
doi:
Substances chimiques
Nerve Tissue Proteins
0
RTN1 protein, human
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
868Références
EBioMedicine. 2016 Dec;14:15-23
pubmed: 28029600
J Mol Biol. 2017 Feb 17;429(4):486-496
pubmed: 28077293
Biochim Biophys Acta. 2015 Mar;1853(3):733-45
pubmed: 25573430
Cell Death Dis. 2013 Apr 04;4:e581
pubmed: 23559015
Autophagy. 2011 Feb;7(2):205-16
pubmed: 21119307
J Neurochem. 2007 Jul;102(2):345-53
pubmed: 17596210
J Pathol. 2010 May;221(1):3-12
pubmed: 20225336
Cell Calcium. 2016 Aug;60(2):74-87
pubmed: 27157108
Elife. 2017 Jun 15;6:
pubmed: 28617241
Int Rev Cytol. 2001;205:149-214
pubmed: 11336391
Autophagy. 2016 Jun 2;12(6):917-35
pubmed: 27123544
Cell. 2006 Feb 10;124(3):573-86
pubmed: 16469703
J Cell Biol. 2008 Sep 8;182(5):911-24
pubmed: 18779370
Autophagy. 2017 Jul 3;13(7):1250-1251
pubmed: 28548902
Mol Pharmacol. 2016 Sep;90(3):371-84
pubmed: 27436127
Autophagy. 2017 May 4;13(5):978-979
pubmed: 28121222
Genome Biol. 2007;8(12):234
pubmed: 18177508
Cell Cycle. 2011 Aug 15;10(16):2635-9
pubmed: 21811092
Cell Death Differ. 2008 Feb;15(2):274-82
pubmed: 18034188
Neuromolecular Med. 2014 Mar;16(1):3-15
pubmed: 24218324
Methods. 2015 Mar;75:87-95
pubmed: 25562747
Oncogene. 2009 Oct 29;28(43):3814-24
pubmed: 19668229
J Cell Physiol. 2017 Nov;232(11):2977-2984
pubmed: 28067409
Cell. 2012 Dec 7;151(6):1256-69
pubmed: 23217709
Cell Death Differ. 2007 Sep;14(9):1576-82
pubmed: 17612585
J Neurochem. 2004 May;89(3):569-80
pubmed: 15086514
Annu Rev Cell Dev Biol. 2009;25:329-54
pubmed: 19575675
EMBO J. 2017 Jun 14;36(12):1719-1735
pubmed: 28495679
Ageing Res Rev. 2017 Mar;34:3-14
pubmed: 27594375
Autophagy. 2015 Nov 2;11(11):1956-1977
pubmed: 26389781
Physiol Rev. 2010 Oct;90(4):1383-435
pubmed: 20959619
Nature. 2015 Jun 18;522(7556):354-8
pubmed: 26040720
J Cell Biol. 2009 Sep 21;186(6):773-82
pubmed: 19797076
Autophagy. 2017 Apr 3;13(4):654-669
pubmed: 28368777