Carbon nanotube-based DNA vaccine against koi herpesvirus given by intramuscular injection.
Carbon nanotubes
DNA vaccine
Immune response
Koi herpes virus
ORF149
Journal
Fish & shellfish immunology
ISSN: 1095-9947
Titre abrégé: Fish Shellfish Immunol
Pays: England
ID NLM: 9505220
Informations de publication
Date de publication:
Mar 2020
Mar 2020
Historique:
received:
30
09
2019
revised:
31
10
2019
accepted:
15
11
2019
pubmed:
20
11
2019
medline:
18
11
2020
entrez:
20
11
2019
Statut:
ppublish
Résumé
Koi herpesvirus (KHV) also named Cyprinid Herpesvirus 3 (CyHV-3) is one of the most threatening pathogens affecting common carp production as well as the valued ornamental koi carp. The current commercial vaccines available are costly and potentially cause severe stress caused by live virus. KHV ORF149 gene has been proved encoding one of the main immunogenic proteins for KHV. In this study, we coupled a plasmid expression vector for ORF149 to single walled carbon nanotubes (SWCNTs) for an anti-KHV vaccine. The vaccine conferred an 81.9% protection against intraperitoneal challenge with KHV. Importantly, SWCNTs as a promising vehicle can enhanced the protective effects 33.9% over that of the naked DNA vaccine at the same dose. The protection was longer and serum antibody production, enzyme activities and immune-related gene expression were all induced in fish vaccinated with the nanotube-DNA vaccine compared with the DNA alone. Thereby, this study demonstrates that the ORF149 DNA vaccine loaded onto SWCNTs as a novel vaccine might provide an effective method of coping with KHV disease using intra-muscular vaccination.
Identifiants
pubmed: 31743761
pii: S1050-4648(19)31078-2
doi: 10.1016/j.fsi.2019.11.035
pii:
doi:
Substances chimiques
Herpesvirus Vaccines
0
Nanotubes, Carbon
0
Vaccines, DNA
0
Types de publication
Journal Article
Randomized Controlled Trial, Veterinary
Langues
eng
Sous-ensembles de citation
IM
Pagination
810-818Informations de copyright
Copyright © 2019 Elsevier Ltd. All rights reserved.