Immunohistochemical analysis of osteopontin expression in oral squamous cell carcinoma.
Moderately differentiated squamous-cell carcinoma
oral squamous cell carcinoma
osteopontin
poorly differentiated squamous-cell carcinoma
well-differentiated squamous cell carcinoma
Journal
Indian journal of dental research : official publication of Indian Society for Dental Research
ISSN: 1998-3603
Titre abrégé: Indian J Dent Res
Pays: India
ID NLM: 9202990
Informations de publication
Date de publication:
Historique:
entrez:
21
11
2019
pubmed:
21
11
2019
medline:
22
11
2019
Statut:
ppublish
Résumé
Oral squamous cell carcinoma (OSCC) is the most common malignant neoplasm of the oral cavity. Osteopontin (OPN) has been proved as a biomarker in varying malignant tumors. Only limited studies detail the role of OPN in OSCC. This study aims to demonstrate the expression of OPN in OSCC and to correlate the expression of OPN with the histologic grades of OSCC. This is a retrospective immunohistochemical study in Dravidian population (linguistically Malayalam). Thirty diagnosed cases of OSCC were subjected to immunohistochemistry using OPN antibody for detection of OPN expression. Ten normal oral mucosal specimens were also stained as controls. Chi-square test and ANOVA followed by Bonferroni test. OPN expression was significantly higher in OSCC patients than in controls. In normal oral mucosal specimens, none of them showed OPN immunoreactivity. A significant difference was observed between total scores and intensities of normal and varying grades of OSCC. A significant difference was also observed between the percentage of positive cells for OPN expression of normal and varying grades of OSCC. However, no significant difference was observed between the percentage of positive cells for OPN expression of well-, moderate-, poorly-differentiated carcinomas. Correlation of OPN expression with lymph node status, site, and sex was found to be statistically insignificant. Insights gained from this study may lead to research targeted at the treatment of OSCC.
Sections du résumé
BACKGROUND
BACKGROUND
Oral squamous cell carcinoma (OSCC) is the most common malignant neoplasm of the oral cavity. Osteopontin (OPN) has been proved as a biomarker in varying malignant tumors. Only limited studies detail the role of OPN in OSCC.
AIMS
OBJECTIVE
This study aims to demonstrate the expression of OPN in OSCC and to correlate the expression of OPN with the histologic grades of OSCC.
SETTINGS AND DESIGN
METHODS
This is a retrospective immunohistochemical study in Dravidian population (linguistically Malayalam).
MATERIALS AND METHODS
METHODS
Thirty diagnosed cases of OSCC were subjected to immunohistochemistry using OPN antibody for detection of OPN expression. Ten normal oral mucosal specimens were also stained as controls.
STATISTICAL ANALYSIS USED
METHODS
Chi-square test and ANOVA followed by Bonferroni test.
RESULTS
RESULTS
OPN expression was significantly higher in OSCC patients than in controls. In normal oral mucosal specimens, none of them showed OPN immunoreactivity. A significant difference was observed between total scores and intensities of normal and varying grades of OSCC. A significant difference was also observed between the percentage of positive cells for OPN expression of normal and varying grades of OSCC. However, no significant difference was observed between the percentage of positive cells for OPN expression of well-, moderate-, poorly-differentiated carcinomas. Correlation of OPN expression with lymph node status, site, and sex was found to be statistically insignificant.
CONCLUSION
CONCLUSIONS
Insights gained from this study may lead to research targeted at the treatment of OSCC.
Identifiants
pubmed: 31745049
pii: IndianJDentRes_2019_30_4_539_271063
doi: 10.4103/ijdr.IJDR_474_17
doi:
Substances chimiques
Osteopontin
106441-73-0
Types de publication
Journal Article
Langues
eng
Pagination
539-543Déclaration de conflit d'intérêts
None