Epigallocatechin 3-gallate attenuates arthritis by regulating Nrf2, HO-1, and cytokine levels in an experimental arthritis model.


Journal

Biotechnology and applied biochemistry
ISSN: 1470-8744
Titre abrégé: Biotechnol Appl Biochem
Pays: United States
ID NLM: 8609465

Informations de publication

Date de publication:
May 2020
Historique:
received: 04 07 2019
accepted: 13 11 2019
pubmed: 21 11 2019
medline: 20 1 2021
entrez: 21 11 2019
Statut: ppublish

Résumé

Epigallocatechin 3-gallate (EGCG) is a polyphenol that has been shown to have antioxidant and anti-inflammatory effects. In this study, collagen-induced arthritis (CIA) model, in Wistar albino rats, was used to elucidate the effect of EGCG on pathogenetic pathways in inflammatory arthritis. The levels of serum TNF-α, IL-17, malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx); the expression levels of tissue heme oxygenase-1 (HO-1) and nuclear factor erythroid 2-related factor 2 (Nrf2); histopathologically, perisynovial inflammation and cartilage-bone destruction were examined. In the sham group, serum TNF-α, IL-17, and MDA levels increased, while SOD, CAT, GPx levels, and the expressions of Nrf2 and HO-1 decreased. On the other hand, in the EGCG administered groups, serum TNF-α, IL-17, and MDA levels improved, while SOD, CAT, GPx levels and the expressions of Nrf2 and HO-1 increased. Moreover, histopathological analysis has shown that perisynovial inflammation and cartilage-bone destruction decreased in the EGCG administered groups. These results suggest that EGCG has an antiarthritic effect by regulating the oxidative-antioxidant balance and cytokine levels in the CIA model, which is a surrogate experimental model of rheumatoid arthritis.

Identifiants

pubmed: 31746064
doi: 10.1002/bab.1860
doi:

Substances chimiques

Collagen Type II 0
Cytokines 0
NF-E2-Related Factor 2 0
Nfe2l2 protein, rat 0
Catechin 8R1V1STN48
epigallocatechin gallate BQM438CTEL
Heme Oxygenase (Decyclizing) EC 1.14.14.18
Hmox1 protein, rat EC 1.14.14.18

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

317-322

Informations de copyright

© 2019 International Union of Biochemistry and Molecular Biology, Inc.

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Auteurs

Ahmet Karatas (A)

Department of Rheumatology, Firat University School of Medicine, Elazig, Turkey.

Adile Ferda Dagli (AF)

Department of Pathology, School of Medicine, Firat University, Elazig, Turkey.

Cemal Orhan (C)

Department of Animal Nutrition, Faculty of Veterinary Science, Firat University, Elazig, Turkey.

Hasan Gencoglu (H)

Department of Biology, Faculty of Science, Firat University, Elazig, Turkey.

Metin Ozgen (M)

Department of Rheumatology, Firat University School of Medicine, Elazig, Turkey.

Nurhan Sahin (N)

Department of Animal Nutrition, Faculty of Veterinary Science, Firat University, Elazig, Turkey.

Kazim Sahin (K)

Department of Animal Nutrition, Faculty of Veterinary Science, Firat University, Elazig, Turkey.

Suleyman Serdar Koca (SS)

Department of Rheumatology, Firat University School of Medicine, Elazig, Turkey.

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Classifications MeSH