Primary osteoblasts, osteoblast precursor cells or osteoblast-like cell lines: Which human cell types are (most) suitable for characterizing 45S5-bioactive glass?
bioactive glass
bone tissue engineering
human mesenchymal stromal cells
human osteoblast-like cells
human primary osteoblasts
Journal
Journal of biomedical materials research. Part A
ISSN: 1552-4965
Titre abrégé: J Biomed Mater Res A
Pays: United States
ID NLM: 101234237
Informations de publication
Date de publication:
03 2020
03 2020
Historique:
received:
02
09
2019
revised:
12
11
2019
accepted:
15
11
2019
pubmed:
21
11
2019
medline:
1
9
2021
entrez:
21
11
2019
Statut:
ppublish
Résumé
The question how bioactive glasses (BGs) influence the viability and osteogenic differentiation of human osteogenic cells has already been addressed by several studies. However, a literature review revealed great differences in the type of cells used for these experiments. Primary human osteoblasts (hOBs) represent the desired standard, but possess the limitation of patient variability and time-consuming isolation protocols. Therefore, several alternative cell types have been used including primary mesenchymal stromal cells (BMSCs) and the "osteoblast-like" cell lines MG-63, Saos-2, HOS, and U2OS. The aim of our study was the identification of the cell type most suitable for tissue engineering projects involving BGs by comparative analysis of cell viability and osteogenic differentiation in response to crystallized 45S5-BG. We observed that hOBs, BMSCs, and MG-63 cells were resistant to 45S5-BG induced cytotoxicity, while the viability of Saos-2, HOS, and U2OS cells was significantly reduced. In addition, we detected alkaline phosphatase activity, except in U2OS cells, that increased upon 45S5-BG cocultivation, demonstrating the induction of osteogenic differentiation. Our data and the fact that the donor-dependent variations can be avoided when using MG-63 cells suggest that these are a promising alternative to primary cells and remain an important cell line for future BG related studies.
Substances chimiques
Biocompatible Materials
0
bioactive glass 45S5
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
663-674Informations de copyright
© 2019 The Authors. Journal of Biomedical Materials Research Part A published by Wiley Periodicals, Inc.
Références
Aina, V., Malavasi, G., Fiorio Pla, A., Munaron, L., & Morterra, C. (2009). Zinc-containing bioactive glasses: Surface reactivity and behaviour towards endothelial cells. Acta Biomaterialia, 5(4), 1211-1222.
Alcaide, M., Portoles, P., Lopez-Noriega, A., Arcos, D., Vallet-Regi, M., & Portoles, M. T. (2010). Interaction of an ordered mesoporous bioactive glass with osteoblasts, fibroblasts and lymphocytes, demonstrating its biocompatibility as a potential bone graft material. Acta Biomaterialia, 6(3), 892-899.
Andrade, Â. L., Valério, P., Goes, A. M., de Fátima Leite, M., & Domingues, R. Z. (2006). Influence of morphology on in vitro compatibility of bioactive glasses. Journal of Non-Crystalline Solids, 352(32), 3508-3511.
Baino, F., Fiorilli, S., Mortera, R., Onida, B., Saino, E., Visai, L., … Vitale-Brovarone, C. (2012). Mesoporous bioactive glass as a multifunctional system for bone regeneration and controlled drug release. Journal of Applied Biomaterials and Functional Materials, 10(1), 12-21.
Baino, F., Hamzehlou, S., & Kargozar, S. (2018). Bioactive glasses: Where are we and where are we going? Journal of Functional Biomaterials, 9(1), 25.
Balasubramanian, P., Büttner, T., Miguez Pacheco, V., & Boccaccini, A. R. (2018). Boron-containing bioactive glasses in bone and soft tissue engineering. Journal of the European Ceramic Society, 38(3), 855-869.
Billiau, A., Edy, V. G., Heremans, H., Van Damme, J., Desmyter, J., Georgiades, J. A., & De Somer, P. (1977). Human interferon: Mass production in a newly established cell line, MG-63. Antimicrobial Agents and Chemotherapy, 12(1), 11-15.
Birmingham, E., Niebur, G. L., McHugh, P. E., Shaw, G., Barry, F. P., & McNamara, L. M. (2012). Osteogenic differentiation of mesenchymal stem cells is regulated by osteocyte and osteoblast cells in a simplified bone niche. European Cells & Materials, 23, 13-27.
Boccardi, E., Philippart, A., Melli, V., Altomare, L., De Nardo, L., Novajra, G., … Boccaccini, A. R. (2016). Bioactivity and mechanical stability of 45S5 bioactive glass scaffolds based on natural marine sponges. Annals of Biomedical Engineering, 44(6), 1881-1893.
Bragdon, B., Burns, R., Baker, A. H., Belkina, A. C., Morgan, E. F., Denis, G. V., … Schlezinger, J. J. (2015). Intrinsic sex-linked variations in osteogenic and Adipogenic differentiation potential of bone marrow multipotent stromal cells. Journal of Cellular Physiology, 230(2), 296-307.
Bretcanu, O., Miola, M., Bianchi, C. L., Marangi, I., Carbone, R., Corazzari, I., … Verne, E. (2017). In vitro biocompatibility of a ferrimagnetic glass-ceramic for hyperthermia application. Materials Science & Engineering. C, Materials for Biological Applications, 73, 778-787.
Bretcanu, O., Misra, S., Roy, I., Renghini, C., Fiori, F., Boccaccini, A. R., & Salih, V. (2009). In vitro biocompatibility of 45S5 bioglass-derived glass-ceramic scaffolds coated with poly(3-hydroxybutyrate). Journal of Tissue Engineering and Regenerative Medicine, 3(2), 139-148.
Chan, Y. H., Lew, W. Z., Lu, E., Loretz, T., Lu, L., Lin, C. T., & Feng, S. W. (2018). An evaluation of the biocompatibility and osseointegration of novel glass fiber reinforced composite implants: in vitro and in vivo studies. Dental Materials, 34(3), 470-485.
Chen, Q. Z., Thompson, I. D., & Boccaccini, A. R. (2006). 45S5 bioglass-derived glass-ceramic scaffolds for bone tissue engineering. Biomaterials, 27(11), 2414-2425.
Ciraldo, F. E., Boccardi, E., Melli, V., Westhauser, F., & Boccaccini, A. R. (2018). Tackling bioactive glass excessive in vitro bioreactivity: Preconditioning approaches for cell culture tests. Acta Biomaterialia, 75, 3-10.
Clover, J., & Gowen, M. (1994). Are MG-63 and HOS TE85 human osteosarcoma cell lines representative models of the osteoblastic phenotype? Bone, 15(6), 585-591.
Czekanska, E. M., Stoddart, M. J., Richards, R. G., & Hayes, J. S. (2012). In search of an osteoblast cell model for in vitro research. European Cells & Materials, 24, 1-17.
Deb, S., Mandegaran, R., & Di Silvio, L. (2010). A porous scaffold for bone tissue engineering/45S5 bioglass derived porous scaffolds for co-culturing osteoblasts and endothelial cells. Journal of Materials Science. Materials in Medicine, 21(3), 893-905.
Deschaseaux, F., Sensebe, L., & Heymann, D. (2009). Mechanisms of bone repair and regeneration. Trends in Molecular Medicine, 15(9), 417-429.
El-Amin, S. F., Botchwey, E., Tuli, R., Kofron, M. D., Mesfin, A., Sethuraman, S., … Laurencin, C. T. (2006). Human osteoblast cells: Isolation, characterization, and growth on polymers for musculoskeletal tissue engineering. Journal of Biomedical Materials Research. Part A, 76(3), 439-449.
Fan, J. P., Kalia, P., Di Silvio, L., & Huang, J. (2014). In vitro response of human osteoblasts to multi-step sol-gel derived bioactive glass nanoparticles for bone tissue engineering. Materials Science & Engineering. C, Materials for Biological Applications, 36, 206-214.
Fernandes, R. J., Harkey, M. A., Weis, M., Askew, J. W., & Eyre, D. R. (2007). The post-translational phenotype of collagen synthesized by SAOS-2 osteosarcoma cells. Bone, 40(5), 1343-1351.
Ferraz, M. P., Knowles, J. C., Olsen, I., Monteiro, F. J., & Santos, J. D. (1999). Flow cytometry analysis of effects of glass on response of osteosarcoma cells to plasma-sprayed hydroxyapatite/CaO-P(2)O(5) coatings. Journal of Biomedical Materials Research, 47(4), 603-611.
Fogh, J., Fogh, J. M., & Orfeo, T. (1977). One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. Journal of the National Cancer Institute, 59(1), 221-226.
Friedl, G., Schmidt, H., Rehak, I., Kostner, G., Schauenstein, K., & Windhager, R. (2007). Undifferentiated human mesenchymal stem cells (hMSCs) are highly sensitive to mechanical strain: Transcriptionally controlled early osteo-chondrogenic response in vitro. Osteoarthritis and Cartilage, 15(11), 1293-1300.
Friedman, M. S., Long, M. W., & Hankenson, K. D. (2006). Osteogenic differentiation of human mesenchymal stem cells is regulated by bone morphogenetic protein-6. Journal of Cellular Biochemistry, 98(3), 538-554.
Fujita, K., Roforth, M. M., Atkinson, E. J., Peterson, J. M., Drake, M. T., McCready, L. K., … Khosla, S. (2013). Isolation and characterization of human osteoblasts from needle biopsies without in vitro culture. Osteoporosis International, 25(3), 887-895. https://doi.org/10.1007/s00198-013-2529-9
Gao, T., Aro, H. T., Ylänen, H., & Vuorio, E. (2001). Silica-based bioactive glasses modulate expression of bone morphogenetic protein-2 mRNA in Saos-2 osteoblasts in vitro. Biomaterials, 22(12), 1475-1483. https://doi.org/10.1016/s0142-9612(00)00288-x
Gong, W. Y., Dong, Y. M., Chen, X. F., & Karabucak, B. (2012). Nano-sized 58S bioactive glass enhances proliferation and osteogenic genes expression of osteoblast-like cells. The Chinese Journal of Dental Research, 15(2), 145-152.
Gough, J. E., Clupper, D. C., & Hench, L. L. (2004). Osteoblast responses to tape-cast and sintered bioactive glass ceramics. Journal of Biomedical Materials Research Part A, 69A(4), 621-628.
Greenspan, D. C. (1999). Bioactive glass: Mechanisms of bone bonding. Tandläkartidningen Årk, 91(8), 1-32.
Hench, L. (2015). Opening paper 2015- some comments on bioglass: Four eras of discovery and development. Biomedical Glasses, 1, 1-11.
Hench, L. L., Splinter, R. J., Allen, W. C., & Greenlee, T. K. (1971). Bonding mechanisms at the interface of ceramic prosthetic materials. Journal of Biomedical Materials Research, 5(6), 117-141.
Hoellig, M., Westhauser, F., Kornienko, K., Xiao, K., Schmidmaier, G., & Moghaddam, A. (2016). Mesenchymal stem cells from reaming material possess high osteogenic potential and react sensitively to bone morphogenetic protein 7. Journal of Applied Biomaterials and Functional Materials, 15, e54-e62.
Hoppe, A., Brandl, A., Bleiziffer, O., Arkudas, A., Horch, R. E., Jokic, B., … Boccaccini, A. R. (2015). In vitro cell response to co-containing 1,393 bioactive glass. Materials Science & Engineering. C, Materials for Biological Applications, 57, 157-163.
Hoppe, A., Guldal, N. S., & Boccaccini, A. R. (2011). A review of the biological response to ionic dissolution products from bioactive glasses and glass-ceramics. Biomaterials, 32(11), 2757-2774.
Huang, J., Jayasinghe, S. N., Best, S. M., Edirisinghe, M. J., Brooks, R. A., Rushton, N., & Bonfield, W. (2005). Novel deposition of nano-sized silicon substituted hydroxyapatite by electrostatic spraying. Journal of Materials Science. Materials in Medicine, 16(12), 1137-1142.
Ikeda, T., Futaesaku, Y., & Tsuchida, N. (1992). In vitro differentiation of the human osteosarcoma cell lines, HOS and KHOS. Virchows Archiv B Cell Pathology, 62(1), 199-206.
Itala, A., Ylanen, H. O., Yrjans, J., Heino, T., Hentunen, T., Hupa, M., & Aro, H. T. (2002). Characterization of microrough bioactive glass surface: Surface reactions and osteoblast responses in vitro. Journal of Biomedical Materials Research, 62(3), 404-411.
Jones, J. R. (2013). Review of bioactive glass: From Hench to hybrids. Acta Biomaterialia, 9(1), 4457-4486.
Jones, J. R., Tsigkou, O., Coates, E. E., Stevens, M. M., Polak, J. M., & Hench, L. L. (2007). Extracellular matrix formation and mineralization on a phosphate-free porous bioactive glass scaffold using primary human osteoblast (HOB) cells. Biomaterials, 28(9), 1653-1663.
Karadjian, M., Essers, C., Tsitlakidis, S., Reible, B., Moghaddam, A., Boccaccini, A. R., & Westhauser, F. (2019). Biological properties of calcium phosphate bioactive glass composite bone substitutes: Current experimental evidence. International Journal of Molecular Sciences, 20(2), 305.
Kargozar, S., Mozafari, M., Hamzehlou, S., Brouki Milan, P., Kim, H.-W., & Baino, F. (2019). Bone tissue engineering using human cells: A comprehensive review on recent trends, current prospects, and recommendations. Applied Sciences, 9(1), 174.
Kaur, G., Pandey, O. P., Singh, K., Homa, D., Scott, B., & Pickrell, G. (2014). A review of bioactive glasses: Their structure, properties, fabrication and apatite formation. Journal of Biomedical Materials Research Part A, 102(1), 254-274.
Kotliarova MS, Zhuikov VA, Chudinova YV, Khaidapova DD, Moisenovich AM, Kon'kov AS, Safonova LA, Bobrova MM, Arkhipova AY, Goncharenko AV and others. Induction of osteogenic differentiation of osteoblast-like cells MG-63 during cultivation on fibroin microcarriers. Moscow University Biological Sciences Bulletin 2016;71(4):212-217.
Lauvrak, S. U., Munthe, E., Kresse, S. H., Stratford, E. W., Namløs, H. M., Meza-Zepeda, L. A., & Myklebost, O. (2013). Functional characterisation of osteosarcoma cell lines and identification of mRNAs and miRNAs associated with aggressive cancer phenotypes. British Journal of Cancer, 109(8), 2228-2236.
Lopes, J. H., Magalhaes, J. A., Gouveia, R. F., Bertran, C. A., Motisuke, M., Camargo, S. E. A., & Triches, E. S. (2016). Hierarchical structures of beta-TCP/45S5 bioglass hybrid scaffolds prepared by gelcasting. Journal of the Mechanical Behavior of Biomedical Materials, 62, 10-23.
Lopes, P. P., Ferreira, B. J., Gomes, P. S., Correia, R. N., Fernandes, M. H., & Fernandes, M. H. (2011). Silicate and borate glasses as composite fillers: A bioactivity and biocompatibility study. Journal of Materials Science. Materials in Medicine, 22(6), 1501-1510.
Luo X, Chen J, Song WX, Tang N, Luo J, Deng ZL, Sharff KA, He G, Bi Y, He BC and others. Osteogenic BMPs promote tumor growth of human osteosarcomas that harbor differentiation defects. Laboratory Investigation 2008;88(12):1264-77.
Mayr-Wohlfart, U., Fiedler, J., Gunther, K. P., Puhl, W., & Kessler, S. (2001). Proliferation and differentiation rates of a human osteoblast-like cell line (SaOS-2) in contact with different bone substitute materials. Journal of Biomedical Materials Research, 57(1), 132-139.
Milkovic, L., Hoppe, A., Detsch, R., Boccaccini, A. R., & Zarkovic, N. (2014). Effects of cu-doped 45S5 bioactive glass on the lipid peroxidation-associated growth of human osteoblast-like cells in vitro. Journal of Biomedical Materials Research. Part A, 102(10), 3556-3561.
Miola M, Brovarone CV, Maina G, Rossi F, Bergandi L, Ghigo D, Saracino S, Maggiora M, Canuto RA, Muzio G and others. In vitro study of manganese-doped bioactive glasses for bone regeneration. Materials Science & Engineering. C, Materials for Biological Applications 2014;38:107-18.
Padilla, S., Sanchez-Salcedo, S., & Vallet-Regi, M. (2005). Bioactive and biocompatible pieces of HA/sol-gel glass mixtures obtained by the gel-casting method. Journal of Biomedical Materials Research. Part A, 75(1), 63-72.
Pérez-Tanoira, R., Kinnari, T. J., Hyyrynen, T., Soininen, A., Pietola, L., Tiainen, V.-M., … Aarnisalo, A. A. (2015). Effects of S53P4 bioactive glass on osteoblastic cell and biomaterial surface interaction. Journal of Materials Science: Materials in Medicine, 26(10). https://doi.org/10.1007/s10856-015-5568-2
Porwal, H., Estili, M., Grunewald, A., Grasso, S., Detsch, R., Hu, C., … Reece, M. J. (2015). 45S5 bioglass([R])-MWCNT composite: Processing and bioactivity. Journal of Materials Science. Materials in Medicine, 26(6), 199.
Price, N., Bendall, S. P., Frondoza, C., Jinnah, R. H., & Hungerford, D. S. (1997). Human osteoblast-like cells (MG63) proliferate on a bioactive glass surface. Journal of Biomedical Materials Research, 37(3), 394-400.
Qazi, T. H., Berkmann, J. C., Schoon, J., Geissler, S., Duda, G. N., Boccaccini, A. R., & Lippens, E. (2018). Dosage and composition of bioactive glasses differentially regulate angiogenic and osteogenic response of human MSCs. Journal of Biomedical Materials Research. Part A, 106(11), 2827-2837.
Qazi, T. H., Hafeez, S., Schmidt, J., Duda, G. N., Boccaccini, A. R., & Lippens, E. (2017). Comparison of the effects of 45S5 and 1393 bioactive glass microparticles on hMSC behavior. Journal of Biomedical Materials Research. Part A, 105(10), 2772-2782.
Raucci, M. G., Adesanya, K., Di Silvio, L., Catauro, M., & Ambrosio, L. (2010). The biocompatibility of silver-containing Na2O.CaO.2SiO2 glass prepared by sol-gel method: in vitro studies. Journal of Biomedical Materials Research. Part B, Applied Biomaterials, 92(1), 102-110.
Reible, B., Schmidmaier, G., Moghaddam, A., & Westhauser, F. (2018). Insulin-like growth Factor-1 as a possible alternative to bone morphogenetic Protein-7 to induce osteogenic differentiation of human mesenchymal stem cells in vitro. International Journal of Molecular Sciences, 19(6), E1674.
Reible, B., Schmidmaier, G., Prokscha, M., Moghaddam, A., & Westhauser, F. (2017). Continuous stimulation with differentiation factors is necessary to enhance osteogenic differentiation of human mesenchymal stem cells in-vitro. Growth Factors, 35(4-5), 179-188.
Rodan, S. B., Imai, Y., Thiede, M. A., Wesolowski, G., Thompson, D., Bar-Shavit, Z., … Rodan, G. A. (1987). Characterization of a human osteosarcoma cell line (Saos-2) with osteoblastic properties. Cancer Research, 47(18), 4961-4966.
Rousseau, J., Lamoureux, F., Picarda, G., Gouin, F., Trichet, V., & Rédini, F. (2010). Chapter 28 - animal models of malignant primary bone tumors and novel therapeutic approaches. In D. Heymann (Ed.), Bone cancer (pp. 333-346). San Diego: Academic Press.
Schneider, C. A., Rasband, W. S., & Eliceiri, K. W. (2012). NIH image to ImageJ: 25 years of image analysis. Nature Methods, 9(7), 671-675.
Thavornyutikarn, B., Feltis, B., Wright, P. F. A., & Turney, T. W. (2019). Effect of pre-treatment of crystallized bioactive glass with cell culture media on structure, degradability, and biocompatibility. Materials Science & Engineering. C, Materials for Biological Applications, 97, 188-197.
Via, A. G., Frizziero, A., & Oliva, F. (2012). Biological properties of mesenchymal stem cells from different sources. Muscles, Ligaments and Tendons Journal, 2(3), 154-162.
Vitale-Brovarone, C., Verne, E., Robiglio, L., Appendino, P., Bassi, F., Martinasso, G., … Canuto, R. (2007). Development of glass-ceramic scaffolds for bone tissue engineering: Characterisation, proliferation of human osteoblasts and nodule formation. Acta Biomaterialia, 3(2), 199-208.
Wang, S., Falk, M. M., Rashad, A., Saad, M. M., Marques, A. C., Almeida, R. M., … Jain, H. (2011). Evaluation of 3D nano-macro porous bioactive glass scaffold for hard tissue engineering. Journal of Materials Science. Materials in Medicine, 22(5), 1195-1203.
Westhauser, F., Ciraldo, F., Balasubramanian, P., Senger, A. S., Schmidmaier, G., Moghaddam, A., & Boccaccini, A. R. (2017). Micro-computed-tomography-guided analysis of in vitro structural modifications in two types of 45S5 bioactive glass based scaffolds. Materials (Basel), 10(12), E1341.
Westhauser, F., Karadjian, M., Essers, C., Senger, A. S., Hagmann, S., Schmidmaier, G., & Moghaddam, A. (2019). Osteogenic differentiation of mesenchymal stem cells is enhanced in a 45S5-supplemented beta-TCP composite scaffold: An in-vitro comparison of Vitoss and Vitoss BA. PLoS One, 14(2), e0212799.
Widholz, B., Tsitlakidis, S., Reible, B., Moghaddam, A., & Westhauser, F. (2019). Pooling of patient-derived mesenchymal stromal cells reduces inter-individual confounder-associated variation without negative impact on cell viability. Proliferation and Osteogenic Differentiation. Cells, 8(6), 633.
Xynos, I. D., Edgar, A. J., Buttery, L. D., Hench, L. L., & Polak, J. M. (2000). Ionic products of bioactive glass dissolution increase proliferation of human osteoblasts and induce insulin-like growth factor II mRNA expression and protein synthesis. Biochemical and Biophysical Research Communications, 276(2), 461-465.
Xynos, I. D., Edgar, A. J., Buttery, L. D., Hench, L. L., & Polak, J. M. (2001). Gene-expression profiling of human osteoblasts following treatment with the ionic products of bioglass 45S5 dissolution. Journal of Biomedical Materials Research, 55(2), 151-157.
Zheng, K., & Boccaccini, A. R. (2017). Sol-gel processing of bioactive glass nanoparticles: A review. Advances in Colloid and Interface Science, 249, 363-373.