PI4KB on Inclusion Bodies Formed by ER Membrane Remodeling Facilitates Replication of Human Parainfluenza Virus Type 3.
1-Phosphatidylinositol 4-Kinase
/ genetics
Endoplasmic Reticulum
/ genetics
Host-Pathogen Interactions
Humans
Inclusion Bodies
/ genetics
Intracellular Membranes
/ metabolism
Parainfluenza Virus 3, Human
/ genetics
Paramyxovirinae
/ genetics
Phosphoproteins
/ metabolism
RNA Viruses
/ genetics
Virus Replication
/ genetics
ER membrane
HPIV3
IBs
PI4KB
Journal
Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691
Informations de publication
Date de publication:
19 11 2019
19 11 2019
Historique:
received:
14
12
2018
revised:
21
06
2019
accepted:
09
10
2019
entrez:
21
11
2019
pubmed:
21
11
2019
medline:
22
9
2020
Statut:
ppublish
Résumé
Many positive-strand RNA viruses remodel the endomembrane to form specialized replication organelles. However, knowledge regarding whether negative-strand RNA viruses take advantage of intracellular membranes for replication is limited. Here we show that a negative-strand RNA virus, human parainfluenza virus type 3 (HPIV3), remodels the endoplasmic reticulum (ER) membrane to form inclusion bodies (IBs), whereby the phosphoprotein (P) of HPIV3 recruits phosphatidylinositol 4-kinase beta (PI4KB) to IBs to generate PI4P, creating a PI4P-enriched microenvironment to promote HPIV3 replication. In addition, we find that human respiratory syncytial virus (HRSV) also takes advantage of the ER to form IBs and that these IBs are also enriched with PI4P. The nucleoprotein of HRSV recruits PI4KB to IBs. These results suggest that paramyxoviruses also exploit the host endomembrane to form IBs and that PI4KB is recruited by viral proteins to enrich IBs with PI4P to facilitate viral replication.
Identifiants
pubmed: 31747597
pii: S2211-1247(19)31366-X
doi: 10.1016/j.celrep.2019.10.052
pmc: PMC7104050
pii:
doi:
Substances chimiques
Phosphoproteins
0
1-Phosphatidylinositol 4-Kinase
EC 2.7.1.67
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2229-2242.e4Informations de copyright
Copyright © 2019 The Author(s). Published by Elsevier Inc. All rights reserved.