Characterization of novel lectins from Burkholderia pseudomallei and Chromobacterium violaceum with seven-bladed β-propeller fold.
Burkholderia pseudomallei
Chromobacterium violaceum
Lectin
Protein structure
Seven-bladed β-propeller fold
Journal
International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578
Informations de publication
Date de publication:
01 Jun 2020
01 Jun 2020
Historique:
received:
15
05
2019
revised:
22
10
2019
accepted:
23
10
2019
pubmed:
22
11
2019
medline:
4
2
2021
entrez:
22
11
2019
Statut:
ppublish
Résumé
Burkholderia pseudomallei and Chromobacterium violaceum are bacteria of tropical and subtropical soil and water that occasionally cause fatal infections in humans and animals. Microbial lectins mediate the adhesion of organisms to host cells, which is the first phase in the development of infection. Here we report the discovery of two novel lectins from the above-mentioned bacteria - BP39L and CV39L. The crystal structures revealed that the lectins possess a seven-bladed β-propeller fold. Functional studies conducted on a series of oligo- and polysaccharides confirmed the preference of BP39L for mannosylated saccharides and CV39L for rather more complex polysaccharides with a monosaccharide preference for β-l-fucose. The presented data indicate that the proteins belong to a currently unknown family of lectins.
Identifiants
pubmed: 31751748
pii: S0141-8130(19)33609-8
doi: 10.1016/j.ijbiomac.2019.10.200
pii:
doi:
Substances chimiques
Bacterial Proteins
0
Lectins
0
Monosaccharides
0
Polysaccharides
0
Fucose
28RYY2IV3F
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1113-1124Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no conflicts of interest with the contents of this article.