Interpretation and Validation of Maximum Absorbance Data Obtained from Turbidimetry Analysis of Plasma Clots.
Journal
Thrombosis and haemostasis
ISSN: 2567-689X
Titre abrégé: Thromb Haemost
Pays: Germany
ID NLM: 7608063
Informations de publication
Date de publication:
Jan 2020
Jan 2020
Historique:
pubmed:
22
11
2019
medline:
7
7
2020
entrez:
22
11
2019
Statut:
ppublish
Résumé
Turbidimetry is used to characterize fibrin clot properties. In purified systems, maximum absorbance (MA) directly relates to fibrin fiber cross-sectional area. However, in plasma samples there are discrepancies in the relationships between MA and fibrinogen concentration, fiber diameter, other clot properties, and cardiovascular disease outcomes, which complicate data interpretation. This study aims to advance understanding of MA of plasma clots through testing how well it relates to fundamental dependence on fibrinogen concentration and fiber diameter as predicted by light scattering theory, other clot properties and lifestyle, and biochemical variables. Plasma samples from 30 apparently healthy individuals with a fibrinogen concentration from 2.4 to 6.4 g/L were included. We performed turbidimetry, permeability, scanning electron microscopy, and rheometry on
Identifiants
pubmed: 31752041
doi: 10.1055/s-0039-1698460
pmc: PMC7369642
mid: NIHMS1606766
doi:
Substances chimiques
Hemostatics
0
Fibrin
9001-31-4
Fibrinogen
9001-32-5
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
44-54Subventions
Organisme : 105700
ID : National Research Foundation
Organisme : NHLBI NIH HHS
ID : R15 HL148842
Pays : United States
Organisme : DM0741
ID : Wake Forest University
Organisme : R15HL148842
ID : National Heart, Lung, and Blood Institute of the National Institutes of Health (U.S.A.)
Organisme : 120070
ID : National Research Foundation
Informations de copyright
Georg Thieme Verlag KG Stuttgart · New York.
Déclaration de conflit d'intérêts
None declared.
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