The Inhibition of MicroRNA-139-5p Promoted Osteoporosis of Bone Marrow-Derived Mesenchymal Stem Cells by Targeting Wnt/Beta-Catenin Signaling Pathway by NOTCH1.
MicroRNA-139-5p
NOTCH1
Wnt
bone marrow-derived mesenchymal stem cell
osteogenic differentiation
β-catenin
Journal
Journal of microbiology and biotechnology
ISSN: 1738-8872
Titre abrégé: J Microbiol Biotechnol
Pays: Korea (South)
ID NLM: 9431852
Informations de publication
Date de publication:
28 Mar 2020
28 Mar 2020
Historique:
pubmed:
23
11
2019
medline:
11
4
2020
entrez:
23
11
2019
Statut:
ppublish
Résumé
We investigated the therapeutic effects of microRNA-139-5p in relation to osteoporosis of bone marrow-derived mesenchymal stem cell (BMSCs) and its underlying mechanisms. In this study we used a dexamethasone-induced in vivo model of osteoporosis and BMSCs were used for the in vitro model. Real-time quantitative polymerase chain reaction (RT-PCR) and gene chip were used to analyze the expression of microRNA-139-5p. In an osteoporosis rat model, the expression of microRNA-139-5p was increased, compared with normal group. Downregulation of microRNA-139-5p promotes cell proliferation and osteogenic differentiation in BMSCs. Especially, up-regulation of microRNA-139-5p reduced cell proliferation and osteogenic differentiation in BMSCs. Overexpression of miR-139-5p induced Wnt/β-catenin and down-regulated NOTCH1 signaling in BMSCs. Down-regulation of miR-139-5p suppressed Wnt/β-catenin and induced NOTCH1 signaling in BMSCs. The inhibition of NOTCH1 reduced the effects of anti-miR-139-5p on cell proliferation and osteogenic differentiation in BMSCs. Activation of Wnt/β-catenin also inhibited the effects of anti-miR-139-5p on cell proliferation and osteogenic differentiation in BMSCs. Taken together, our results suggested that the inhibition of microRNA-139-5p promotes osteogenic differentiation of BMSCs via targeting Wnt/β-catenin signaling pathway by NOTCH1.
Identifiants
pubmed: 31752063
pii: 10.4014/jmb.1908.08036
doi: 10.4014/jmb.1908.08036
pmc: PMC9728286
doi:
Substances chimiques
CTNNB1 protein, mouse
0
MIRN139 microRNA, mouse
0
MicroRNAs
0
Notch1 protein, mouse
0
Receptor, Notch1
0
beta Catenin
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
448-458Références
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