Production and characterization of virus-like particles of grapevine fanleaf virus presenting L2 epitope of human papillomavirus minor capsid protein.
Bionanoparticles
HPV
Heterologous expression systems
Nepovirus
Protein refolding
Vaccine antigen
Journal
BMC biotechnology
ISSN: 1472-6750
Titre abrégé: BMC Biotechnol
Pays: England
ID NLM: 101088663
Informations de publication
Date de publication:
21 11 2019
21 11 2019
Historique:
received:
12
07
2019
accepted:
09
10
2019
entrez:
23
11
2019
pubmed:
23
11
2019
medline:
9
4
2020
Statut:
epublish
Résumé
Virus-like particle (VLP) platform represents a promising approach for the generation of efficient and immunogenic subunit vaccines. Here, the feasibility of using grapevine fanleaf virus (GFLV) VLPs as a new carrier for the presentation of human papillomavirus (HPV) L2 epitope was studied. To achieve this goal, a model of the HPV L2 epitope secondary structure was predicted and its insertion within 5 external loops in the GFLV capsid protein (CP) was evaluated. The epitope sequence was genetically inserted in the αB-αB The results demonstrate that GFLV VLPs constitute a potential scaffold for surface display of the epitope of interest.
Sections du résumé
BACKGROUND
Virus-like particle (VLP) platform represents a promising approach for the generation of efficient and immunogenic subunit vaccines. Here, the feasibility of using grapevine fanleaf virus (GFLV) VLPs as a new carrier for the presentation of human papillomavirus (HPV) L2 epitope was studied. To achieve this goal, a model of the HPV L2 epitope secondary structure was predicted and its insertion within 5 external loops in the GFLV capsid protein (CP) was evaluated.
RESULTS
The epitope sequence was genetically inserted in the αB-αB
CONCLUSIONS
The results demonstrate that GFLV VLPs constitute a potential scaffold for surface display of the epitope of interest.
Identifiants
pubmed: 31752839
doi: 10.1186/s12896-019-0566-y
pii: 10.1186/s12896-019-0566-y
pmc: PMC6868843
doi:
Substances chimiques
Capsid Proteins
0
Epitopes
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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