Force interactions between Yersiniae lipopolysaccharides and monoclonal antibodies: An optical tweezers study.
Lipopolysaccharide
Microspheres
Monoclonal antibodies
Optical tweezers
Yersinia pseudotuberculosis
Journal
Journal of biomechanics
ISSN: 1873-2380
Titre abrégé: J Biomech
Pays: United States
ID NLM: 0157375
Informations de publication
Date de publication:
23 01 2020
23 01 2020
Historique:
received:
26
04
2019
revised:
06
11
2019
accepted:
07
11
2019
pubmed:
23
11
2019
medline:
12
11
2020
entrez:
23
11
2019
Statut:
ppublish
Résumé
This article reports the force spectroscopy investigation of interactions between lipopolysaccharides (LPSs) of two species from Yersinia genus and complementary (or heterologous) monoclonal antibodies (mAbs). We have obtained the experimental data by optical trapping on the "sensitized polystyrene microsphere - sensitized glass substrate" model system at its approach - retraction in vertical plane. We detected non-specific interactions in low-amplitude areas on histograms mainly due to physicochemical properties of abiotic surface and specific interactions in complementary pairs "antigen - antibodies" in high-amplitude areas (100-120 pN) on histograms. The developed measurement procedure can be used for detection of rupture forces in other molecular pairs.
Identifiants
pubmed: 31753213
pii: S0021-9290(19)30758-4
doi: 10.1016/j.jbiomech.2019.109504
pii:
doi:
Substances chimiques
Antibodies, Monoclonal
0
Lipopolysaccharides
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
109504Informations de copyright
Copyright © 2019 Elsevier Ltd. All rights reserved.