Evaluation of the automated BD Phoenix CPO Detect test for detection and classification of carbapenemases in Gram negatives.
Anti-Bacterial Agents
/ pharmacology
Automation
Bacterial Proteins
/ biosynthesis
Belgium
Enterobacteriaceae
/ classification
Gram-Negative Bacteria
/ classification
Gram-Negative Bacterial Infections
/ diagnosis
Humans
Microbial Sensitivity Tests
/ methods
Molecular Typing
/ methods
Reproducibility of Results
Sensitivity and Specificity
beta-Lactamases
/ biosynthesis
Ambler classification
BD CPO detect test
Carbapenemase detection
Carbapenemase producing organism
Gram-negative
Phoenix
Journal
Diagnostic microbiology and infectious disease
ISSN: 1879-0070
Titre abrégé: Diagn Microbiol Infect Dis
Pays: United States
ID NLM: 8305899
Informations de publication
Date de publication:
Feb 2020
Feb 2020
Historique:
received:
29
07
2019
revised:
02
10
2019
accepted:
02
10
2019
pubmed:
23
11
2019
medline:
10
6
2020
entrez:
23
11
2019
Statut:
ppublish
Résumé
We evaluated the performance of the automated BD Phoenix CPO Detect test (BD-CPO test) for detection and Ambler classification of carbapenemases in Enterobacteriaceae, P.aeruginosa and A.baumannii complex. A collection of 287 Gram-negative clinical isolates, with a reduced susceptibility to at least one carbapenem including 184 carbapenemase-producing organisms (CPO) and 103 non-CPO, was tested. The BD-CPO test showed an overall sensitivity of 89.7% and specificity of 83.5% for carbapenemase detection. 1/7 of class A, 82.9% of class B, and 89.8% of class D carbapenemases were correctly classified. Poor detection sensitivity of 68.9% and specificity of 62.1% in P.aeruginosa was observed. However, combination with ceftazidime/avibactam susceptibility, provided by this panel, increased the performances for P.aeruginosa. The integration of an automated carbapenemase detection and classification in routine susceptibility panels would save time and help for therapeutic management. Further developments are needed to improve the accuracy of the BD-CPO test.
Identifiants
pubmed: 31753520
pii: S0732-8893(19)30764-3
doi: 10.1016/j.diagmicrobio.2019.114911
pii:
doi:
Substances chimiques
Anti-Bacterial Agents
0
Bacterial Proteins
0
beta-Lactamases
EC 3.5.2.6
carbapenemase
EC 3.5.2.6
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
114911Informations de copyright
Copyright © 2019 Elsevier Inc. All rights reserved.