IL-10 induces MC3T3-E1 cells differentiation towards osteoblastic fate in murine model.
Fracture
IGF1R
IL-10
mRNA
miRNA
osteoblast
Journal
Journal of cellular and molecular medicine
ISSN: 1582-4934
Titre abrégé: J Cell Mol Med
Pays: England
ID NLM: 101083777
Informations de publication
Date de publication:
01 2020
01 2020
Historique:
received:
07
09
2019
revised:
04
10
2019
accepted:
28
10
2019
pubmed:
23
11
2019
medline:
4
5
2021
entrez:
23
11
2019
Statut:
ppublish
Résumé
Interleukin-10 (IL-10) displays well-documented anti-inflammatory effects, but its effects on osteoblast differentiation have not been investigated. In this study, we found IL-10 negatively regulates microRNA-7025-5p (miR-7025-5p), the down-regulation of which enhances osteoblast differentiation. Furthermore, through luciferase reporter assays, we found evidence that insulin-like growth factor 1 receptor (IGF1R) is a miR-7025-5p target gene that positively regulates osteoblast differentiation. In vivo studies indicated that the pre-injection of IL-10 leads to increased bone formation, while agomiR-7025-5p injection delays fracture healing. Taken together, these results indicate that IL-10 induces osteoblast differentiation via regulation of the miR-7025-5p/IGF1R axis. IL-10 therefore represents a promising therapeutic strategy to promote fracture healing.
Identifiants
pubmed: 31755174
doi: 10.1111/jcmm.14832
pmc: PMC6933380
doi:
Substances chimiques
IL10 protein, mouse
0
MicroRNAs
0
Interleukin-10
130068-27-8
Receptor, IGF Type 1
EC 2.7.10.1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1076-1086Commentaires et corrections
Type : ErratumIn
Informations de copyright
© 2019 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.
Références
Cells. 2019 Apr 08;8(4):
pubmed: 30965609
Mol Ther. 2014 May;22(5):999-1007
pubmed: 24496384
Cell Mol Life Sci. 2014 Dec;71(24):4747-61
pubmed: 25108446
J Biol Chem. 1991 Nov 5;266(31):21044-50
pubmed: 1657946
Clin Cancer Res. 2019 Feb 15;25(4):1343-1357
pubmed: 30397176
J Clin Invest. 2018 Dec 3;128(12):5251-5266
pubmed: 30352426
Pharmacol Res. 2019 Jun;144:292-305
pubmed: 31048033
Bone Res. 2018 Feb 26;6:5
pubmed: 29507819
Mol Ther. 2017 Oct 4;25(10):2254-2269
pubmed: 28807569
J Cell Biol. 2012 May 14;197(4):509-21
pubmed: 22564414
Cell Death Differ. 2019 Dec;26(12):2652-2666
pubmed: 31076633
J Exp Med. 2011 Aug 29;208(9):1849-61
pubmed: 21844205
J Exp Med. 2002 Aug 19;196(4):541-9
pubmed: 12186845
Bone. 2019 Nov;128:115034
pubmed: 31421252
Stem Cells. 2019 Aug;37(8):1030-1041
pubmed: 31021484
J Cell Mol Med. 2019 May;23(5):3683-3695
pubmed: 30907511
Connect Tissue Res. 1995;31(4):279-82
pubmed: 15612368
J Biol Chem. 2012 Jun 22;287(26):21926-35
pubmed: 22544738
J Biol Chem. 1994 Mar 25;269(12):9392-6
pubmed: 8132679
Nat Med. 2019 Jan;25(1):75-81
pubmed: 30478422
Cancer Lett. 2019 Aug 1;456:59-68
pubmed: 31042587
Cell Death Dis. 2019 May 10;10(5):375
pubmed: 31076571
Nat Commun. 2018 Nov 14;9(1):4772
pubmed: 30429466
Mol Ther. 2019 May 8;27(5):999-1016
pubmed: 30852139
J Am Coll Surg. 2019 Sep;229(3):305-315
pubmed: 31034883
Circ Res. 2005 Mar 4;96(4):398-400
pubmed: 15692088
Proc Natl Acad Sci U S A. 2018 Feb 27;115(9):2204-2209
pubmed: 29440427
J Exp Med. 2007 Feb 19;204(2):285-97
pubmed: 17283207
J Clin Invest. 2019 Apr 4;129(6):2578-2594
pubmed: 30946695
Nature. 2018 Oct;562(7725):133-139
pubmed: 30250253
Mol Cancer Ther. 2019 May;18(5):929-936
pubmed: 30926641
Cell Death Differ. 2019 Oct;26(10):2029-2045
pubmed: 30728459
PLoS Biol. 2018 Nov 13;16(11):e2006522
pubmed: 30422989
J Cell Mol Med. 2020 Jan;24(1):1076-1086
pubmed: 31755174
Biomaterials. 2015 Jun;53:239-50
pubmed: 25890723
Eur Heart J. 2019 Aug 7;40(30):2482-2491
pubmed: 30698710
Proc Natl Acad Sci U S A. 2011 Apr 12;108(15):6139-44
pubmed: 21444814
Biochem Biophys Res Commun. 1991 Mar 15;175(2):577-82
pubmed: 1902096
J Cell Mol Med. 2019 Sep;23(9):6140-6153
pubmed: 31304676
Nat Genet. 2019 Apr;51(4):716-727
pubmed: 30833796
J Biol Chem. 2006 Nov 17;281(46):35088-96
pubmed: 16982608