Electrochemical detection of different p53 conformations by using nanostructured surfaces.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
22 11 2019
22 11 2019
Historique:
received:
07
04
2019
accepted:
07
11
2019
entrez:
24
11
2019
pubmed:
24
11
2019
medline:
4
11
2020
Statut:
epublish
Résumé
Protein electrochemistry represents a powerful technique for investigating the function and structure of proteins. Currently available biochemical assays provide limited information related to the conformational state of proteins and high costs. This work provides novel insights into the electrochemical investigation of the metalloprotein p53 and its redox products using label-free direct electrochemistry and label-based antibody-specific approaches. First, the redox activities of different p53 redox products were qualitatively investigated on carbon-based electrodes. Then, focusing on the open p53 isoform (denatured p53), a quantitative analysis was performed, comparing the performances of different bulk and nanostructured materials (carbon and platinum). Overall, four different p53 products could be successfully discriminated, from wild type to denatured. Label-free analysis suggested a single electron exchange with electron transfer rate constants on the order of 1 s
Identifiants
pubmed: 31758050
doi: 10.1038/s41598-019-53994-6
pii: 10.1038/s41598-019-53994-6
pmc: PMC6874615
doi:
Substances chimiques
Antibodies
0
TP53 protein, human
0
Tumor Suppressor Protein p53
0
Platinum
49DFR088MY
Carbon
7440-44-0
Gold
7440-57-5
Types de publication
Comparative Study
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
17347Références
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