MicroRNA profiling reveals important functions of miR-125b and let-7a during human retinal pigment epithelial cell differentiation.
Cell Differentiation
/ physiology
Cell Line
Flow Cytometry
Gene Expression Profiling
Human Embryonic Stem Cells
/ metabolism
Humans
Immunohistochemistry
MicroRNAs
/ genetics
Microscopy, Electron, Transmission
Microscopy, Fluorescence
Phagocytosis
/ physiology
Real-Time Polymerase Chain Reaction
Retinal Pigment Epithelium
/ cytology
Signal Transduction
Embryonic stem cells
Retinal pigment epithelium
Small RNA sequencing
let-7
miR-125b
microRNA
Journal
Experimental eye research
ISSN: 1096-0007
Titre abrégé: Exp Eye Res
Pays: England
ID NLM: 0370707
Informations de publication
Date de publication:
01 2020
01 2020
Historique:
received:
22
10
2018
revised:
20
10
2019
accepted:
19
11
2019
pubmed:
24
11
2019
medline:
18
7
2020
entrez:
24
11
2019
Statut:
ppublish
Résumé
Retinal pigment epithelial (RPE) cells are indispensable for eye organogenesis and vision. To realize the therapeutic potential of in vitro-generated RPE cells for cell-replacement therapy of RPE-related retinopathies, molecular mechanisms of RPE specification and maturation need to be investigated. So far, many attempts have been made to decipher the regulatory networks involved in the differentiation of human pluripotent stem cells into RPE cells. Here, we exploited a highly-efficient RPE differentiation protocol to determine global expression patterns of microRNAs (miRNAs) during human embryonic stem cell (hESC) differentiation into RPE using small RNA sequencing. Our results revealed a significant downregulation of pluripotency-associated miRNAs along with a significant upregulation of RPE-associated miRNAs in differentiating cells. Our functional analyses indicated that two RPE-enriched miRNAs (i.e. miR-125b and let-7a) could promote RPE fate at the expense of neural fate during RPE differentiation. Taken together, these mechanistic interrogations might shed light on a better understanding of RPE cell development and provide insights for the future application of these cells in regenerative medicine.
Identifiants
pubmed: 31758976
pii: S0014-4835(18)30777-2
doi: 10.1016/j.exer.2019.107883
pii:
doi:
Substances chimiques
MIRN125 microRNA, human
0
MicroRNAs
0
mirnlet7 microRNA, human
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
107883Informations de copyright
Copyright © 2019 Elsevier Ltd. All rights reserved.