PACS1 is an HIV-1 cofactor that functions in Rev-mediated nuclear export of viral RNA.
Cell Line
Cell Nucleus
/ metabolism
Cytoplasm
/ metabolism
HIV Infections
/ metabolism
HIV-1
/ physiology
Host-Pathogen Interactions
Humans
Karyopherins
/ metabolism
Protein Binding
Protein Transport
RNA Transport
RNA, Viral
/ metabolism
Receptors, Cytoplasmic and Nuclear
/ metabolism
Vesicular Transport Proteins
/ metabolism
Virus Replication
rev Gene Products, Human Immunodeficiency Virus
/ metabolism
Exportin 1 Protein
HIV
PACS
RNA export
Rev
Journal
Virology
ISSN: 1096-0341
Titre abrégé: Virology
Pays: United States
ID NLM: 0110674
Informations de publication
Date de publication:
15 01 2020
15 01 2020
Historique:
received:
05
03
2019
revised:
09
10
2019
accepted:
15
10
2019
pubmed:
24
11
2019
medline:
8
9
2020
entrez:
24
11
2019
Statut:
ppublish
Résumé
HIV-1 is dependent upon cellular proteins to mediate the many processes required for viral replication. One such protein, PACS1, functions to localize Furin to the trans-Golgi network where Furin cleaves HIV-1 gp160 Envelope into gp41 and gp120. We show here that PACS1 also shuttles between the nucleus and cytoplasm, associates with the viral Rev protein and its cofactor CRM1, and contributes to nuclear export of viral transcripts. PACS1 appears specific to the Rev-CRM1 pathway and not other retroviral RNA export pathways. Over-expression of PACS1 increases nuclear export of unspliced viral RNA and significantly increases p24 expression in HIV-1-infected Jurkat CD4
Identifiants
pubmed: 31759187
pii: S0042-6822(19)30293-4
doi: 10.1016/j.virol.2019.10.004
pmc: PMC7335006
mid: NIHMS1604114
pii:
doi:
Substances chimiques
Karyopherins
0
PACS1 protein, human
0
RNA, Viral
0
Receptors, Cytoplasmic and Nuclear
0
Vesicular Transport Proteins
0
rev Gene Products, Human Immunodeficiency Virus
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
88-96Subventions
Organisme : NIAID NIH HHS
ID : R21 AI114335
Pays : United States
Organisme : NIAID NIH HHS
ID : R33 AI116167
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI050237
Pays : United States
Organisme : NIAID NIH HHS
ID : R21 AI116167
Pays : United States
Organisme : NIAID NIH HHS
ID : P30 AI036211
Pays : United States
Informations de copyright
Copyright © 2019 Elsevier Inc. All rights reserved.