Potential of Treated Dentin Matrix Xenograft for Dentin-Pulp Tissue Engineering.

Animals Cattle Dental Pulp Dentin / transplantation Heterografts Microscopy, Electron, Scanning Peptides Rats Tissue Engineering
Atelopeptidization demineralization dentin-pulp complex regeneration treated dentin matrix xenograft

Journal

Journal of endodontics
ISSN: 1878-3554
Titre abrégé: J Endod
Pays: United States
ID NLM: 7511484

Informations de publication

Date de publication:
Jan 2020
Historique:
received: 09 05 2019
revised: 30 09 2019
accepted: 04 10 2019
pubmed: 25 11 2019
medline: 28 1 2020
entrez: 25 11 2019
Statut: ppublish

Résumé

This study aims to develop and characterize the regenerative potential of an atelopeptidized treated dentin matrix xenograft using in vitro and in vivo models. Freshly extracted bovine dentin was pulverized into 250- to 500-μm particles and demineralized with 17% EDTA for 1, 7, and 13 days. The samples were atelopeptidized with pepsin. The degree of demineralization and the effect of atelopeptidization were assessed using field emission scanning electron microscopy combined with energy-dispersive X-ray spectroscopy and Fourier transform infrared spectroscopy, respectively. The expression of dentin matrix acidic phosphoprotein 1, dentin sialophosphoprotein, and osteopontin was evaluated in dental pulp stem cells using quantitative real-time polymerase chain reaction. The samples were then implanted intramuscularly in rats for 30 days, and the inflammatory cells were quantified histologically. Field emission scanning electron microscopy combined with energy-dispersive X-ray spectroscopy revealed an exposed tubular structure of dentin after 1 and 7 days of demineralization. Fourier transform infrared spectroscopy confirmed the absence of amide peaks at 1260 to 1640/cm after atelopeptidization. The dental pulp stem cell expression of dentin matrix acidic phosphoprotein 1 and dentin sialophosphoprotein increased in all compared with the untreated control group (P < .05). The maximum expression rates were observed for the 1-day demineralized and atelopeptidized group. The 1-day demineralized group elicited the highest inflammatory response compared with the 7- or 13-day demineralized groups (P < .001). Atelopeptidization significantly decreased the inflammatory response only in the 1-day demineralized dentin group (P < .05). Atelopeptidization of 1-day demineralized dentin xenograft preserved the collagen structure, minimized the immune reaction, and provided sufficient regenerative potential.

Identifiants

DOI: 10.1016/j.joen.2019.10.005 PMID: 31759677
pubmed: 31759677
pii: S0099-2399(19)30741-1
doi: 10.1016/j.joen.2019.10.005
pii:
doi:

Substances chimiques

Peptides 0

Types de publication

Journal Article

Langues

eng

Pagination

57-64.e1

Informations de copyright

Copyright © 2019 American Association of Endodontists. Published by Elsevier Inc. All rights reserved.

Auteurs

Hengameh Bakhtiar (H)

Department of Endodontics, Faculty of Dentistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran; Faculty of Dentistry, University of Toronto, Toronto, Ontario, Canada; Stem Cell Research Center, Tissue Engineering and Regenerative Medicine Institute, Tehran Central Branch, Islamic Azad University, Tehran, Iran.

Amir Mazidi (A)

Student Research Committee, Faculty of Dentistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.

Saeed Mohammadi-Asl (S)

Student Research Committee, Faculty of Dentistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.

Sadegh Hasannia (S)

Department of Clinical Biochemistry, Tarbiat Modarres University, Tehran, Iran.

Mohammad Reza Ellini (MR)

Student Research Committee, Faculty of Dentistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran.

Mohammad Pezeshki-Modaress (M)

Burn Research Center, Iran University of Medical Sciences, Tehran, Iran.

Seyed Naser Ostad (SN)

Department of Toxicology-Pharmacology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.

Kerstin Galler (K)

Department of Conservative Dentistry and Periodontology, University Hospital Regensburg, Regensburg, Germany.

Amir Azarpazhooh (A)

Faculty of Dentistry, University of Toronto, Toronto, Ontario, Canada; Department of Dentistry, Mount Sinai Hospital, Toronto, Ontario, Canada; Clinical Epidemiology and Health Care Research, Institute of Health Policy, Management and Evaluation, University of Toronto, Toronto, Ontario, Canada.

Anil Kishen (A)

Faculty of Dentistry, University of Toronto, Toronto, Ontario, Canada; Department of Dentistry, Mount Sinai Hospital, Toronto, Ontario, Canada. Electronic address: Anil.Kishen@dentistry.utoronto.ca.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Potential of Treated Dentin Matrix Xenograft...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Artiodactyla › Ruminants › Bovins › Potential of Treated Dentin Matrix Xenograft...
questionsmedicales.fr › Système stomatognathique › Bouche › Denture › Composants dentaires › Pulpe dentaire › Potential of Treated Dentin Matrix Xenograft...
questionsmedicales.fr › Système stomatognathique › Bouche › Denture › Composants dentaires › Dentine › Potential of Treated Dentin Matrix Xenograft...
questionsmedicales.fr › Régions du corps › Transplants › Hétérogreffes › Potential of Treated Dentin Matrix Xenograft...
questionsmedicales.fr › Techniques d'investigation › Microscopie électronique › Microscopie électronique à balayage › Potential of Treated Dentin Matrix Xenograft...
questionsmedicales.fr › Acides aminés, peptides et protéines › Peptides › Potential of Treated Dentin Matrix Xenograft...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Rodentia › Muridae › Murinae › Rats › Potential of Treated Dentin Matrix Xenograft...
questionsmedicales.fr › Technologie, industrie et agriculture › Ingénierie › Bioingénierie › Ingénierie cellulaire › Ingénierie tissulaire › Potential of Treated Dentin Matrix Xenograft...