HNF4 Regulates Fatty Acid Oxidation and Is Required for Renewal of Intestinal Stem Cells in Mice.
Gene Expression
Mitochondria
Regulatory Networks
Stemness
Journal
Gastroenterology
ISSN: 1528-0012
Titre abrégé: Gastroenterology
Pays: United States
ID NLM: 0374630
Informations de publication
Date de publication:
03 2020
03 2020
Historique:
received:
11
07
2019
revised:
22
10
2019
accepted:
15
11
2019
pubmed:
25
11
2019
medline:
29
4
2020
entrez:
25
11
2019
Statut:
ppublish
Résumé
Functions of intestinal stem cells (ISCs) are regulated by diet and metabolic pathways. Hepatocyte nuclear factor 4 (HNF4) family are transcription factors that bind fatty acids. We investigated how HNF4 transcription factors regulate metabolism and their functions in ISCs in mice. We performed studies with Villin-Cre Crypt cells rapidly absorbed labeled fatty acids, and messenger RNA levels of Lgr5 In mice, the transcription factors HNF4A and HNF4G regulate the expression of genes required for FAO and are required for renewal of ISCs.
Sections du résumé
BACKGROUND & AIMS
Functions of intestinal stem cells (ISCs) are regulated by diet and metabolic pathways. Hepatocyte nuclear factor 4 (HNF4) family are transcription factors that bind fatty acids. We investigated how HNF4 transcription factors regulate metabolism and their functions in ISCs in mice.
METHODS
We performed studies with Villin-Cre
RESULTS
Crypt cells rapidly absorbed labeled fatty acids, and messenger RNA levels of Lgr5
CONCLUSIONS
In mice, the transcription factors HNF4A and HNF4G regulate the expression of genes required for FAO and are required for renewal of ISCs.
Identifiants
pubmed: 31759926
pii: S0016-5085(19)41587-4
doi: 10.1053/j.gastro.2019.11.031
pmc: PMC7062567
mid: NIHMS1545425
pii:
doi:
Substances chimiques
Fabp2 protein, mouse
0
Fatty Acid-Binding Proteins
0
Fatty Acids
0
Hepatocyte Nuclear Factor 4
0
Hnf4g protein, mouse
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
985-999.e9Subventions
Organisme : NCI NIH HHS
ID : P30 CA072720
Pays : United States
Organisme : NIDDK NIH HHS
ID : U24 DK085532
Pays : United States
Organisme : NIDDK NIH HHS
ID : U01 DK103141
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA190558
Pays : United States
Organisme : NIDDK NIH HHS
ID : U01 DK085532
Pays : United States
Organisme : NCCIH NIH HHS
ID : R01 AT010243
Pays : United States
Informations de copyright
Copyright © 2020 AGA Institute. Published by Elsevier Inc. All rights reserved.
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