An immunocompetent rat model of infection with Cryptosporidium hominis and Cryptosporidium parvum.
Animals
Antibodies, Protozoan
/ biosynthesis
Antigens, Protozoan
Cryptosporidiosis
/ prevention & control
Cryptosporidium
/ immunology
Cryptosporidium parvum
/ immunology
Female
Immunity, Humoral
Immunocompetence
Models, Animal
Rats
Rats, Sprague-Dawley
/ immunology
Sporozoites
/ immunology
Trachea
/ parasitology
Vaccination
/ methods
Animal model
Cryptosporidiosis
Immunity
Intratracheal infection
Sporozoites
Vaccine
Journal
International journal for parasitology
ISSN: 1879-0135
Titre abrégé: Int J Parasitol
Pays: England
ID NLM: 0314024
Informations de publication
Date de publication:
01 2020
01 2020
Historique:
received:
24
07
2019
revised:
06
10
2019
accepted:
10
10
2019
pubmed:
25
11
2019
medline:
6
6
2020
entrez:
25
11
2019
Statut:
ppublish
Résumé
A major obstacle to developing vaccines against cryptosporidiosis, a serious diarrheal disease of children in developing countries, is the lack of rodent models essential to identify and screen protective immunogens. Rodent models commonly used for drug discovery are unsuitable for vaccine development because they either are purposefully immunodeficient or immunosuppressed. Here, we describe the development and optimization of an immunocompetent intratracheal (IT) rat model susceptible to infections with sporozoites of Cryptosporidium parvum and Cryptosporidium hominis - the primary causes of human cryptosporidiosis. A model suitable for screening of parasite immunogens is a prerequisite for immunogen screening and vaccine development.
Identifiants
pubmed: 31759945
pii: S0020-7519(19)30268-1
doi: 10.1016/j.ijpara.2019.10.002
pii:
doi:
Substances chimiques
Antibodies, Protozoan
0
Antigens, Protozoan
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
19-22Subventions
Organisme : NIAID NIH HHS
ID : U19 AI109776
Pays : United States
Informations de copyright
Copyright © 2019. Published by Elsevier Ltd.