Towards standardization of immune functional assays.
Host response
Immune functional assay
Independent validation
Standardization
Stimulation
Journal
Clinical immunology (Orlando, Fla.)
ISSN: 1521-7035
Titre abrégé: Clin Immunol
Pays: United States
ID NLM: 100883537
Informations de publication
Date de publication:
01 2020
01 2020
Historique:
received:
29
07
2019
revised:
16
10
2019
accepted:
18
11
2019
pubmed:
25
11
2019
medline:
1
8
2020
entrez:
25
11
2019
Statut:
ppublish
Résumé
Recent advances in the immunotherapy field require evaluation of the immune function to adapt therapeutic decisions. Immune functional assays (IFA) are able to reveal the immune status and would be useful to further adapt and/or improve patient's care. However, standardized methods are needed to implement IFA in clinical settings. We carried out an independent validation of a published method used to characterize the underlying host response to infectious conditions using an IFA. We evaluated the reproducibility and robustness of this IFA and the associated readout using an independent healthy volunteers (HV) cohort. Expression of a 44-gene signature and IFNγ protein secretion was assessed after stimulation. We observed a strong host-response correlation between the two cohorts. We also highlight that standardized methods for immune function evaluation exist and could be implemented in larger-scale studies. This IFA could be a relevant tool to reveal innate and adaptive immune dysfunction in immune-related disorders patients.
Identifiants
pubmed: 31760096
pii: S1521-6616(19)30405-X
doi: 10.1016/j.clim.2019.108312
pii:
doi:
Substances chimiques
Interferon-gamma
82115-62-6
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
108312Informations de copyright
Copyright © 2019 The Authors. Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest William Mouton, Chloé Albert Vega, Guy Oriol, Alexandre Pachot, Julien Textoris, François Mallet and Karen Brengel-Pesce, are employed at bioMérieux, an in-vitro diagnostic company. François Bartolo is an employee of Soladis, a services company specialized in data-related projects management.