Multiday maintenance of extracorporeal lungs using cross-circulation with conscious swine.
acute lung injury
airway lavage
alveolar recruitment
bronchoalveolar lavage fluid
chimerism
cross-circulation
ex vivo lung perfusion
extracorporeal membrane oxygenation
infrared thermography
lung bioengineering
lung transplantation
medical thermography
normothermic organ perfusion
organ shortage
regenerative medicine
swine model
tissue engineering
transplantation
whole organ bioreactor
Journal
The Journal of thoracic and cardiovascular surgery
ISSN: 1097-685X
Titre abrégé: J Thorac Cardiovasc Surg
Pays: United States
ID NLM: 0376343
Informations de publication
Date de publication:
04 2020
04 2020
Historique:
received:
21
06
2019
revised:
05
09
2019
accepted:
09
09
2019
pubmed:
26
11
2019
medline:
4
4
2020
entrez:
26
11
2019
Statut:
ppublish
Résumé
Lung remains the least-utilized solid organ for transplantation. Efforts to recover donor lungs with reversible injuries using ex vivo perfusion systems are limited to <24 hours of support. Here, we demonstrate the feasibility of extending normothermic extracorporeal lung support to 4 days using cross-circulation with conscious swine. A swine behavioral training program and custom enclosure were developed to enable multiday cross-circulation between extracorporeal lungs and recipient swine. Lungs were ventilated and perfused in a normothermic chamber for 4 days. Longitudinal analyses of extracorporeal lungs (ie, functional assessments, multiscale imaging, cytokine quantification, and cellular assays) and recipient swine (eg, vital signs and blood and tissue analyses) were performed. Throughout 4 days of normothermic support, extracorporeal lung function was maintained (arterial oxygen tension/inspired oxygen fraction >400 mm Hg; compliance >20 mL/cm H We demonstrate feasibility of normothermic maintenance of extracorporeal lungs for 4 days by cross-circulation with conscious swine. Cross-circulation approaches could support the recovery of damaged lungs and enable organ bioengineering to improve transplant outcomes.
Identifiants
pubmed: 31761338
pii: S0022-5223(19)32146-4
doi: 10.1016/j.jtcvs.2019.09.121
pmc: PMC7094131
mid: NIHMS1065507
pii:
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
1640-1653.e18Subventions
Organisme : NHLBI NIH HHS
ID : L30 HL143733
Pays : United States
Organisme : NHLBI NIH HHS
ID : U01 HL134760
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL120046
Pays : United States
Organisme : NIBIB NIH HHS
ID : P41 EB027062
Pays : United States
Organisme : NHLBI NIH HHS
ID : T32 HL007854
Pays : United States
Commentaires et corrections
Type : CommentIn
Type : CommentIn
Type : CommentIn
Informations de copyright
Copyright © 2019 The American Association for Thoracic Surgery. Published by Elsevier Inc. All rights reserved.
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