Biological membranes in EV biogenesis, stability, uptake, and cargo transfer: an ISEV position paper arising from the ISEV membranes and EVs workshop.
Exosomes
Extracellular vesicles
ISEV workshop
biogenesis
fusion
membranes
position paper
technology
uptake
Journal
Journal of extracellular vesicles
ISSN: 2001-3078
Titre abrégé: J Extracell Vesicles
Pays: United States
ID NLM: 101610479
Informations de publication
Date de publication:
2019
2019
Historique:
received:
03
06
2019
revised:
23
08
2019
accepted:
04
10
2019
entrez:
26
11
2019
pubmed:
26
11
2019
medline:
26
11
2019
Statut:
epublish
Résumé
Paracrine and endocrine roles have increasingly been ascribed to extracellular vesicles (EVs) generated by multicellular organisms. Central to the biogenesis, content, and function of EVs are their delimiting lipid bilayer membranes. To evaluate research progress on membranes and EVs, the International Society for Extracellular Vesicles (ISEV) conducted a workshop in March 2018 in Baltimore, Maryland, USA, bringing together key opinion leaders and hands-on researchers who were selected on the basis of submitted applications. The workshop was accompanied by two scientific surveys and covered four broad topics: EV biogenesis and release; EV uptake and fusion; technologies and strategies used to study EV membranes; and EV transfer and functional assays. In this ISEV position paper, we synthesize the results of the workshop and the related surveys to outline important outstanding questions about EV membranes and describe areas of consensus. The workshop discussions and survey responses reveal that while much progress has been made in the field, there are still several concepts that divide opinion. Good consensus exists in some areas, including particular aspects of EV biogenesis, uptake and downstream signalling. Areas with little to no consensus include EV storage and stability, as well as whether and how EVs fuse with target cells. Further research is needed in these key areas, as a better understanding of membrane biology will contribute substantially towards advancing the field of extracellular vesicles.
Identifiants
pubmed: 31762963
doi: 10.1080/20013078.2019.1684862
pii: 1684862
pmc: PMC6853251
doi:
Types de publication
Journal Article
Langues
eng
Pagination
1684862Subventions
Organisme : NIMH NIH HHS
ID : R21 MH118164
Pays : United States
Organisme : NIDA NIH HHS
ID : R01 DA047807
Pays : United States
Organisme : NIA NIH HHS
ID : R56 AG057430
Pays : United States
Organisme : NIDA NIH HHS
ID : R01 DA040385
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL124187
Pays : United States
Organisme : NIH HHS
ID : T32 OD011089
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL148786
Pays : United States
Organisme : NCI NIH HHS
ID : UG3 CA241694
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL140469
Pays : United States
Organisme : NIA NIH HHS
ID : T32 AG052375
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI144997
Pays : United States
Informations de copyright
© 2019 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group on behalf of The International Society for Extracellular Vesicles.
Références
Curr Biol. 2011 Dec 6;21(23):1951-9
pubmed: 22100064
J Control Release. 2015 Feb 28;200:87-96
pubmed: 25555362
Nat Med. 2000 Jul;6(7):769-75
pubmed: 10888925
Sci Rep. 2017 Mar 14;7:41623
pubmed: 28290538
Nat Biotechnol. 2019 Mar;37(3):314-322
pubmed: 30778230
J Thromb Haemost. 2014 Jul;12(7):1182-92
pubmed: 24818656
Nat Cell Biol. 2010 Jan;12(1):19-30; sup pp 1-13
pubmed: 19966785
Methods Mol Biol. 2017;1660:233-241
pubmed: 28828661
PLoS One. 2014 Dec 03;9(12):e113651
pubmed: 25470250
Blood. 2005 Jul 1;106(1):216-23
pubmed: 15790784
Cell Death Differ. 2011 Jul;18(7):1161-73
pubmed: 21252909
Anal Chem. 2018 Jul 3;90(13):7855-7861
pubmed: 29870225
EMBO Rep. 2015 Feb;16(2):213-20
pubmed: 25568329
J Biol Chem. 2003 May 30;278(22):20083-90
pubmed: 12639953
J Extracell Vesicles. 2014 Aug 04;3:
pubmed: 25143819
Exp Mol Med. 2019 Mar 15;51(3):1-12
pubmed: 30872574
Int J Mol Med. 2014 May;33(5):1055-63
pubmed: 24573178
Traffic. 2005 Feb;6(2):131-43
pubmed: 15634213
Nat Cell Biol. 2009 Sep;11(9):1143-9
pubmed: 19684575
Proc Natl Acad Sci U S A. 2014 Aug 5;111(31):E3234-42
pubmed: 24938788
Proc Natl Acad Sci U S A. 2012 Mar 13;109(11):4146-51
pubmed: 22315426
Nanoscale. 2018 Feb 1;10(5):2413-2426
pubmed: 29334397
Prog Lipid Res. 2005 Jul;44(4):207-34
pubmed: 15979148
J Biol Chem. 2011 Sep 2;286(35):30911-30925
pubmed: 21737841
EMBO Rep. 2016 Aug;17(8):1184-203
pubmed: 27402548
Neuron. 2013 Mar 20;77(6):1039-46
pubmed: 23522040
J Extracell Vesicles. 2017 Mar 7;6(1):1286095
pubmed: 28326170
Cardiovasc Res. 2016 Jun 1;110(3):319-30
pubmed: 26980205
J Extracell Vesicles. 2015 Apr 20;4:26316
pubmed: 25899407
Nanoscale. 2018 Jan 18;10(3):881-906
pubmed: 29265147
J Control Release. 2016 Feb 28;224:77-85
pubmed: 26773767
Blood. 2004 Nov 15;104(10):3257-66
pubmed: 15284116
Dev Cell. 2019 Feb 25;48(4):573-589.e4
pubmed: 30745143
J Immunol. 2016 Oct 15;197(8):3382-3392
pubmed: 27619994
Science. 2008 Feb 29;319(5867):1244-7
pubmed: 18309083
J Lipid Res. 2019 Jan;60(1):9-18
pubmed: 30076207
Sci Rep. 2016 Sep 21;6:33702
pubmed: 27650365
Elife. 2016 Sep 22;5:
pubmed: 27657169
J Am Chem Soc. 2009 May 13;131(18):6452-9
pubmed: 19415935
Clin Cancer Res. 2001 Dec;7(12):4109-14
pubmed: 11751509
J Magn Reson. 2010 Nov;207(1):89-94
pubmed: 20851650
J Extracell Vesicles. 2015 Dec 23;4:29509
pubmed: 26700615
Biophys J. 2009 Sep 2;97(5):1371-80
pubmed: 19720025
Blood. 2009 Feb 26;113(9):1977-81
pubmed: 19064723
FEBS J. 2018 Aug;285(16):3114-3133
pubmed: 29953723
PLoS One. 2009;4(4):e5219
pubmed: 19381331
Biochim Biophys Acta. 1981 Jul 6;645(1):63-70
pubmed: 6266476
Neurobiol Dis. 2019 Oct;130:104512
pubmed: 31229685
J Magn Reson. 2014 Feb;239:9-15
pubmed: 24355622
Biochemistry. 1987 Aug 25;26(17):5389-97
pubmed: 3676258
J Biol Chem. 1987 Jul 5;262(19):9412-20
pubmed: 3597417
J Biol Chem. 2009 Dec 4;284(49):34211-22
pubmed: 19801663
FEBS Lett. 2015 Jun 4;589(13):1391-8
pubmed: 25937124
J Immunol. 2000 Aug 1;165(3):1259-65
pubmed: 10903724
Nat Biotechnol. 2011 Apr;29(4):341-5
pubmed: 21423189
J Extracell Vesicles. 2017 Jun 20;6(1):1329476
pubmed: 28717422
Front Immunol. 2018 May 24;9:1011
pubmed: 29881375
J Extracell Vesicles. 2014 Dec 22;3:26913
pubmed: 25536934
Nat Cell Biol. 2018 Mar;20(3):332-343
pubmed: 29459780
Nat Commun. 2017 Jan 09;8:14041
pubmed: 28067230
Thromb Haemost. 2018 Sep;118(9):1612-1624
pubmed: 30112749
J Cell Biol. 2010 Apr 19;189(2):223-32
pubmed: 20404108
J Extracell Vesicles. 2015 Dec 31;4:30087
pubmed: 26725829
Biophys J. 2008 Mar 1;94(5):1699-706
pubmed: 18024495
Philos Trans R Soc Lond B Biol Sci. 2018 Jan 5;373(1737):
pubmed: 29158309
Biochem Biophys Res Commun. 2017 Feb 19;483(4):1178-1186
pubmed: 27659705
Sci Rep. 2016 May 10;6:25613
pubmed: 27161857
Nat Protoc. 2016 Jan;11(1):87-101
pubmed: 26658469
Nat Methods. 2017 Feb 28;14(3):228-232
pubmed: 28245209
Nature. 2017 Jun 22;546(7659):498-503
pubmed: 28607485
J Control Release. 2017 Nov 28;266:100-108
pubmed: 28919558
J Immunol. 2008 Jun 15;180(12):8146-52
pubmed: 18523279
Curr Protoc Cell Biol. 2006 Apr;Chapter 3:Unit 3.22
pubmed: 18228490
Nat Commun. 2013;4:2980
pubmed: 24356509
Expert Rev Anticancer Ther. 2011 Sep;11(9):1341-3
pubmed: 21929308
J Extracell Vesicles. 2018 Mar 13;7(1):1446660
pubmed: 29696074
Bioeng Transl Med. 2017 Jun 26;2(2):170-179
pubmed: 28932818
J Thromb Haemost. 2018 Jul;16(7):1423-1436
pubmed: 29781099
Nanoscale. 2017 Sep 21;9(36):13693-13706
pubmed: 28880029
Trends Cell Biol. 2015 Jun;25(6):364-72
pubmed: 25683921
Nature. 2015 Nov 19;527(7578):329-35
pubmed: 26524530
J Cell Biol. 2013 Feb 18;200(4):373-83
pubmed: 23420871
Nat Commun. 2015 May 13;6:7029
pubmed: 25967391
Nanoscale. 2018 May 10;10(18):8547-8559
pubmed: 29693684
Nat Commun. 2017 Sep 27;8(1):709
pubmed: 28955033
J Biol Chem. 2016 Jan 22;291(4):1652-1663
pubmed: 26601950
Anal Chem. 2015 Apr 21;87(8):4168-76
pubmed: 25674701
J Biol Chem. 2013 Jun 14;288(24):17713-24
pubmed: 23653359
Cytometry A. 2016 Feb;89(2):135-47
pubmed: 25688721
Nat Rev Mol Cell Biol. 2018 Apr;19(4):213-228
pubmed: 29339798
J Cell Biol. 1983 Aug;97(2):329-39
pubmed: 6309857
Biochim Biophys Acta. 2014 Dec;1838(12):3137-3144
pubmed: 25195712
PLoS One. 2012;7(3):e34308
pubmed: 22479596
J Cell Sci. 2013 Dec 15;126(Pt 24):5553-65
pubmed: 24105262
Cancer Prev Res (Phila). 2012 Mar;5(3):492-497
pubmed: 22158052
Proc Natl Acad Sci U S A. 2003 Jun 24;100(13):7626-31
pubmed: 12802020
J Cell Biol. 2015 Oct 12;211(1):27-37
pubmed: 26459596
Nanoscale. 2019 Jan 23;11(4):1531-1537
pubmed: 30623961
ACS Appl Mater Interfaces. 2016 Oct 5;8(39):25825-25833
pubmed: 27617891
Nat Rev Rheumatol. 2014 Jun;10(6):356-64
pubmed: 24535546
PLoS One. 2011;6(9):e24145
pubmed: 21909417
Proteomics. 2018 Sep;18(17):e1800266
pubmed: 30035390
Cell. 2014 Oct 23;159(3):635-46
pubmed: 25307933
Elife. 2018 Dec 17;7:
pubmed: 30556811
Blood. 1999 Dec 1;94(11):3791-9
pubmed: 10572093
Nat Struct Mol Biol. 2008 Jul;15(7):690-8
pubmed: 18596815
Proc Natl Acad Sci U S A. 2016 Feb 23;113(8):E968-77
pubmed: 26858453
J Extracell Vesicles. 2017 Sep 26;6(1):1378056
pubmed: 29184623
J Cell Biol. 2016 Jul 18;214(2):197-213
pubmed: 27402952
Stem Cells. 2007 Sep;25(9):2245-56
pubmed: 17556595
Cell Discov. 2016 Jun 21;2:16011
pubmed: 27462458
Sci Rep. 2016 Apr 13;6:24120
pubmed: 27071676
Sci Rep. 2016 Aug 09;6:31175
pubmed: 27503761
PLoS One. 2015 Mar 23;10(3):e0121184
pubmed: 25798862
Am J Pathol. 2006 Dec;169(6):2127-36
pubmed: 17148675
Glia. 2015 Apr;63(4):626-34
pubmed: 25451814
EMBO J. 2009 Apr 22;28(8):1043-54
pubmed: 19300439
ACS Nano. 2014 Jan 28;8(1):483-494
pubmed: 24383518
J Cell Biol. 2016 Apr 25;213(2):173-84
pubmed: 27114500
Nat Commun. 2017 Oct 26;8(1):1145
pubmed: 29074968
J Biol Chem. 2013 Apr 26;288(17):11649-61
pubmed: 23463506
Nat Rev Immunol. 2009 Aug;9(8):581-93
pubmed: 19498381
Clin Lung Cancer. 2009 Jan;10(1):42-6
pubmed: 19289371
Blood. 2003 Dec 15;102(13):4336-44
pubmed: 12881314
Cell Rep. 2016 May 3;15(5):978-987
pubmed: 27117408
J Biol Chem. 2005 Aug 5;280(31):28439-50
pubmed: 15870081
Oncotarget. 2017 Feb 28;8(9):14395-14407
pubmed: 28122335
J Extracell Vesicles. 2019 Mar 27;8(1):1593755
pubmed: 30949310
J Extracell Vesicles. 2018 Nov 23;7(1):1535750
pubmed: 30637094
Cell Adh Migr. 2017 Mar 4;11(2):135-150
pubmed: 27689411
Biotechniques. 2016 Jan 01;60(1):35-41
pubmed: 26757810
Lab Chip. 2017 Oct 25;17(21):3558-3577
pubmed: 28832692
Magn Reson Med. 2015 Jul;74(1):266-271
pubmed: 25052384
Hum Mol Genet. 2012 Oct 15;21(R1):R125-34
pubmed: 22872698
Nat Rev Cardiol. 2017 May;14(5):259-272
pubmed: 28150804
Nat Cell Biol. 2019 Jan;21(1):9-17
pubmed: 30602770
FEBS J. 2014 May;281(9):2214-27
pubmed: 24605801
EMBO Mol Med. 2016 Oct 4;8(10):1162-1183
pubmed: 27596437
Adv Drug Deliv Rev. 2013 Mar;65(3):357-67
pubmed: 22820532
Nat Med. 2012 Jun;18(6):883-91
pubmed: 22635005
Immunity. 2019 Mar 19;50(3):738-750.e7
pubmed: 30770248
Cell Rep. 2013 Dec 12;5(5):1159-68
pubmed: 24290760
Biochim Biophys Acta Biomembr. 2018 Jun;1860(6):1350-1361
pubmed: 29551275
Nature. 2015 Jul 9;523(7559):177-82
pubmed: 26106858
Biochim Biophys Acta. 2015 Sep;1848(9):1783-95
pubmed: 25839353
J Cell Biol. 2018 Mar 5;217(3):1129-1142
pubmed: 29339438
J Extracell Vesicles. 2016 Feb 24;5:29828
pubmed: 26928672
Cell. 2018 Jan 11;172(1-2):262-274.e11
pubmed: 29328915
Traffic. 2010 May;11(5):675-87
pubmed: 20136776
J Control Release. 2017 Feb 10;247:127-133
pubmed: 28065862
Electrophoresis. 2011 Aug;32(15):1976-83
pubmed: 21557262
Cell Mol Neurobiol. 2016 Apr;36(3):301-12
pubmed: 27053351
J Extracell Vesicles. 2013 May 27;2:
pubmed: 24009894
Anticancer Res. 2014 Jul;34(7):3419-23
pubmed: 24982349
J Exp Med. 1996 Mar 1;183(3):1161-72
pubmed: 8642258
Chem Commun (Camb). 2018 Jun 26;54(52):7219-7222
pubmed: 29900459
J Biol Chem. 2002 Nov 8;277(45):43216-23
pubmed: 12205095
Biochemistry. 2011 Oct 25;50(42):8983-5
pubmed: 21936505
Nat Commun. 2019 Aug 2;10(1):3490
pubmed: 31375709
Cell Mol Life Sci. 2011 Aug;68(16):2667-88
pubmed: 21560073
Sci Rep. 2016 Nov 08;6:36338
pubmed: 27821849
Nat Cell Biol. 2012 Oct;14(10):1036-45
pubmed: 22983114
J Extracell Vesicles. 2016 Oct 31;5:32945
pubmed: 27802845
ACS Chem Biol. 2013 Jan 18;8(1):218-25
pubmed: 23075500
J Control Release. 2014 Dec 10;195:72-85
pubmed: 25094032
Nat Protoc. 2012 Jun 14;7(7):1311-26
pubmed: 22722367
Platelets. 2017 May;28(3):256-262
pubmed: 28277059
Nat Protoc. 2020 Jan;15(1):102-121
pubmed: 31836866
Proc Natl Acad Sci U S A. 2018 Feb 6;115(6):E1127-E1136
pubmed: 29367422
J Control Release. 2018 Mar 10;273:180-183
pubmed: 29360476