Colonisation of the proximal intestinal remnant in newborn infants with enterostomy: a longitudinal study protocol.
Bacteroidetes
/ classification
DNA, Bacterial
/ isolation & purification
Digestive System Abnormalities
/ microbiology
Enterocolitis, Necrotizing
/ microbiology
Enterostomy
Gastrointestinal Microbiome
Humans
Infant, Newborn
Infant, Premature
Intensive Care Units, Neonatal
Intestinal Perforation
/ microbiology
Intestines
/ microbiology
Longitudinal Studies
Portugal
Prospective Studies
RNA, Ribosomal, 16S
/ genetics
Research Design
congenital malformations of the gastrointestinal tract
dysbiosis
enterostomy
microbiota
necrotizing enterocolitis
newborn infants
Journal
BMJ open
ISSN: 2044-6055
Titre abrégé: BMJ Open
Pays: England
ID NLM: 101552874
Informations de publication
Date de publication:
24 11 2019
24 11 2019
Historique:
entrez:
27
11
2019
pubmed:
27
11
2019
medline:
3
11
2020
Statut:
epublish
Résumé
The gut microbiota plays a main role in the maintenance of host's health. Exposure to different conditions in early life contributes to distinct 'pioneer' bacterial communities in the intestine, which shape the newborn infant development. Newborn infants with congenital malformations of the gastrointestinal tract (CMGIT), necrotising enterocolitis (NEC) and spontaneous intestinal perforation (SIP) commonly require abdominal surgery and enterostomy. The knowledge about the colonisation of these newborns' intestine by microorganisms is scarce. This protocol is designed to explore the microbial colonisation over time of the proximal intestinal remnant in newborn infants who underwent surgery for CMGIT, NEC or SIP and require enterostomy. The literature about microbiota colonisation in newborn infants with enterostomy was reviewed and an observational, longitudinal, prospective study was designed. The infants will be recruited at the Neonatal Intensive Care Unit of the Hospital Dona Estefânia, Centro Hospitalar Universitário de Lisboa Central. Samples of the enterostomy effluent will be collected every 3 days, through 21 days after the first collection. The microorganisms colonising the proximal intestinal remnant will be identified using the 16S rRNA sequence analysis and a subset of microorganisms will be quantified using real-time PCR. This protocol may serve as basis for future observational and interventional studies on the modulation of the intestinal microbiota (eg, probiotics) on short and long-term outcomes in this population. This study protocol was approved by the Ethics Committee of Centro Hospitalar Universitário de Lisboa Central (441/2017) and by the Ethics Committee of NOVA Medical School, Universidade Nova de Lisboa (n°50/2018/CEFCM). The results will be spread through peer-reviewed publications and presentations at international scientific meetings. NCT03340259.
Identifiants
pubmed: 31767579
pii: bmjopen-2019-028916
doi: 10.1136/bmjopen-2019-028916
pmc: PMC6886948
doi:
Substances chimiques
DNA, Bacterial
0
RNA, Ribosomal, 16S
0
Banques de données
ClinicalTrials.gov
['NCT03340259']
Types de publication
Journal Article
Observational Study
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e028916Informations de copyright
© Author(s) (or their employer(s)) 2019. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.
Déclaration de conflit d'intérêts
Competing interests: None declared.
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