Microtubule and Actin Differentially Regulate Synaptic Vesicle Cycling to Maintain High-Frequency Neurotransmission.
Actin Cytoskeleton
/ physiology
Actins
/ physiology
Animals
Auditory Pathways
/ physiology
Brain Stem
/ cytology
Bridged Bicyclo Compounds, Heterocyclic
/ pharmacology
Excitatory Postsynaptic Potentials
/ drug effects
Exocytosis
/ physiology
Female
Male
Microtubules
/ physiology
Presynaptic Terminals
/ physiology
Rats
Rats, Wistar
Synaptic Transmission
/ drug effects
Synaptic Vesicles
/ physiology
Thiazolidines
/ pharmacology
Trapezoid Body
/ physiology
Vinblastine
/ pharmacology
F-actin
microtubules
neurotransmission
short-term depression
synaptic vesicles
Journal
The Journal of neuroscience : the official journal of the Society for Neuroscience
ISSN: 1529-2401
Titre abrégé: J Neurosci
Pays: United States
ID NLM: 8102140
Informations de publication
Date de publication:
02 01 2020
02 01 2020
Historique:
received:
03
07
2019
revised:
23
10
2019
accepted:
16
11
2019
pubmed:
27
11
2019
medline:
21
7
2020
entrez:
27
11
2019
Statut:
ppublish
Résumé
Cytoskeletal filaments such as microtubules (MTs) and filamentous actin (F-actin) dynamically support cell structure and functions. In central presynaptic terminals, F-actin is expressed along the release edge and reportedly plays diverse functional roles, but whether axonal MTs extend deep into terminals and play any physiological role remains controversial. At the calyx of Held in rats of either sex, confocal and high-resolution microscopy revealed that MTs enter deep into presynaptic terminal swellings and partially colocalize with a subset of synaptic vesicles (SVs). Electrophysiological analysis demonstrated that depolymerization of MTs specifically prolonged the slow-recovery time component of EPSCs from short-term depression induced by a train of high-frequency stimulation, whereas depolymerization of F-actin specifically prolonged the fast-recovery component. In simultaneous presynaptic and postsynaptic action potential recordings, depolymerization of MTs or F-actin significantly impaired the fidelity of high-frequency neurotransmission. We conclude that MTs and F-actin differentially contribute to slow and fast SV replenishment, thereby maintaining high-frequency neurotransmission.
Identifiants
pubmed: 31767677
pii: JNEUROSCI.1571-19.2019
doi: 10.1523/JNEUROSCI.1571-19.2019
pmc: PMC6939482
doi:
Substances chimiques
Actins
0
Bridged Bicyclo Compounds, Heterocyclic
0
Thiazolidines
0
Vinblastine
5V9KLZ54CY
latrunculin A
SRQ9WWM084
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
131-142Informations de copyright
Copyright © 2020 Piriya Ananda Babu, Wang et al.
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