Effects of a DPP4 Inhibitor on Progression of NASH-related HCC and the p62/ Keap1/Nrf2-Pentose Phosphate Pathway in a Mouse Model.

Antidiabetic agent Dipeptidyl peptidase-4 Hepatoma Nonalcoholic fatty liver disease Pentose phosphate shunt

Journal

Liver cancer
ISSN: 2235-1795
Titre abrégé: Liver Cancer
Pays: Switzerland
ID NLM: 101597993

Informations de publication

Date de publication:
Oct 2019
Historique:
received: 13 01 2018
accepted: 29 06 2018
entrez: 27 11 2019
pubmed: 27 11 2019
medline: 27 11 2019
Statut: ppublish

Résumé

Diabetes mellitus is a risk factor for hepatocellular carcinoma (HCC) in patients with nonalcoholic steatohepatitis (NASH). Dipeptidyl peptidase-4 inhibitor (DPP4i), an antidiabetic agent, is reported to affect cell proliferation. We aimed to investigate the effects of DPP4i on the progression of NASH-related HCC and its metabolic pathway in a mouse model. A mouse model of NASH-related HCC was used in this study. Eight-week-old mice were administered either DPP4i (sitagliptin 30 mg/kg/day; DPP4i group; The number and volume of HCC were significantly lower in the DPP4i group than in the control group (1.8 ± 1.2 vs. 4.5 ± 1.7/liver, We demonstrated that DDP4i prevented the progression of NASH-related HCC in a mouse model. Furthermore, metabolome analysis revealed that DDP4i downregulated the pentose phosphate pathway with suppression of the p62/Keap1/Nrf2 pathway. Thus, DDP4i may prevent tumor progression through inhibition of metabolic reprogramming in NASH-related HCC.

Sections du résumé

BACKGROUND AND AIMS OBJECTIVE
Diabetes mellitus is a risk factor for hepatocellular carcinoma (HCC) in patients with nonalcoholic steatohepatitis (NASH). Dipeptidyl peptidase-4 inhibitor (DPP4i), an antidiabetic agent, is reported to affect cell proliferation. We aimed to investigate the effects of DPP4i on the progression of NASH-related HCC and its metabolic pathway in a mouse model.
METHODS METHODS
A mouse model of NASH-related HCC was used in this study. Eight-week-old mice were administered either DPP4i (sitagliptin 30 mg/kg/day; DPP4i group;
RESULTS RESULTS
The number and volume of HCC were significantly lower in the DPP4i group than in the control group (1.8 ± 1.2 vs. 4.5 ± 1.7/liver,
CONCLUSIONS CONCLUSIONS
We demonstrated that DDP4i prevented the progression of NASH-related HCC in a mouse model. Furthermore, metabolome analysis revealed that DDP4i downregulated the pentose phosphate pathway with suppression of the p62/Keap1/Nrf2 pathway. Thus, DDP4i may prevent tumor progression through inhibition of metabolic reprogramming in NASH-related HCC.

Identifiants

pubmed: 31768345
doi: 10.1159/000491763
pii: lic-0008-0359
pmc: PMC6873068
doi:

Types de publication

Journal Article

Langues

eng

Pagination

359-372

Informations de copyright

Copyright © 2018 by S. Karger AG, Basel.

Déclaration de conflit d'intérêts

All authors disclose no conflicts.

Références

Int J Mol Sci. 2015 Dec 08;16(12):29207-18
pubmed: 26670228
Oncol Rep. 2016 Feb;35(2):676-82
pubmed: 26573958
J Clin Endocrinol Metab. 2017 Aug 1;102(8):2930-2940
pubmed: 28575350
Mol Cell. 2013 Sep 12;51(5):618-31
pubmed: 24011591
Hepatology. 2016 Jan;63(1):173-84
pubmed: 26403645
Am J Gastroenterol. 2014 Mar;109(3):325-34
pubmed: 24445574
Hepatology. 2011 Sep 2;54(3):1063-70
pubmed: 21563202
BMC Syst Biol. 2012 Nov 10;6:139
pubmed: 23140568
J Clin Diagn Res. 2015 Dec;9(12):FC14-6
pubmed: 26816911
Hepatology. 2016 Jul;64(1):73-84
pubmed: 26707365
Drugs Today (Barc). 2007 Nov;43(11):801-14
pubmed: 18174966
Hepatology. 2005 Jun;41(6):1313-21
pubmed: 15915461
Cell Death Dis. 2017 Feb 9;8(2):e2603
pubmed: 28182015
Nat Cell Biol. 2010 Mar;12(3):213-23
pubmed: 20173742
Diabetes. 2011 Apr;60(4):1246-57
pubmed: 21330637
Tumour Biol. 2016 Oct 7;:null
pubmed: 27718126
J Hepatol. 1997 Aug;27(2):337-45
pubmed: 9288609
Int J Cancer. 2013 Nov 15;133(10):2498-503
pubmed: 23649733
Hepatology. 2010 Jun;51(6):1972-8
pubmed: 20209604
Dis Markers. 2017;2017:7062517
pubmed: 28260828
Sci Rep. 2016 Jun 08;6:27557
pubmed: 27272163
Liver Int. 2010 Mar;30(3):479-86
pubmed: 20040053
Clin Exp Metastasis. 2015 Oct;32(7):677-87
pubmed: 26233333
Sci Transl Med. 2016 Apr 13;8(334):334ra51
pubmed: 27075625
Behav Brain Res. 2001 Nov 1;125(1-2):279-84
pubmed: 11682119
World J Gastroenterol. 2013 Apr 21;19(15):2298-306
pubmed: 23613622
Diabetes Res Clin Pract. 2014 Jul;105(1):47-57
pubmed: 24842243
Oncotarget. 2017 Jan 31;8(5):8679-8692
pubmed: 28060721
Int J Mol Med. 2017 Apr;39(4):969-983
pubmed: 28260070
Clin Gastroenterol Hepatol. 2012 Dec;10(12):1342-1359.e2
pubmed: 23041539
J Gastroenterol. 2015 Mar;50(3):333-41
pubmed: 24912965
Science. 2014 Oct 24;346(6208):477-81
pubmed: 25342805
Hepatology. 1999 Jan;29(1):205-16
pubmed: 9862868
Am J Pathol. 2004 Nov;165(5):1499-508
pubmed: 15509521
Sci Rep. 2018 Feb 5;8(1):2362
pubmed: 29402900
Am J Physiol Endocrinol Metab. 2013 Oct 15;305(8):E987-98
pubmed: 23964070
Cancer Cell. 2012 Jul 10;22(1):66-79
pubmed: 22789539
Eur J Pharm Sci. 2017 Mar 30;100:17-24
pubmed: 28065853
J Biol Chem. 2001 Jul 27;276(30):28554-61
pubmed: 11373280
Adv Exp Med Biol. 2000;477:167-71
pubmed: 10849744
Endocrinology. 2013 Mar;154(3):1260-70
pubmed: 23337530
Exp Anim. 2018 May 10;67(2):201-218
pubmed: 29276215
Cell Mol Gastroenterol Hepatol. 2017 May 31;4(2):303-323.e1
pubmed: 28840186
Br J Pharmacol. 2015 Nov;172(21):5096-109
pubmed: 26267432
Nat Genet. 2002 Dec;32 Suppl:496-501
pubmed: 12454644
Med Mol Morphol. 2013 Sep;46(3):141-52
pubmed: 23430399
Am J Gastroenterol. 2012 Feb;107(2):253-61
pubmed: 22008893
Metabolomics. 2010 Mar;6(1):78-95
pubmed: 20300169
Biol Rev Camb Philos Soc. 2015 Aug;90(3):927-63
pubmed: 25243985

Auteurs

Takumi Kawaguchi (T)

Division of Gastroenterology, Department of Medicine, Kurume University School of Medicine, Kurume, Japan.

Dan Nakano (D)

Division of Gastroenterology, Department of Medicine, Kurume University School of Medicine, Kurume, Japan.

Hironori Koga (H)

Division of Gastroenterology, Department of Medicine, Kurume University School of Medicine, Kurume, Japan.
Liver Cancer Division, Research Center for Innovative Cancer Therapy, Kurume University, Kurume, Japan.

Takuji Torimura (T)

Division of Gastroenterology, Department of Medicine, Kurume University School of Medicine, Kurume, Japan.
Liver Cancer Division, Research Center for Innovative Cancer Therapy, Kurume University, Kurume, Japan.

Classifications MeSH