Reconstitution of the Human Nuclear RNA Exosome.
Cell Nucleus
/ metabolism
Endoribonucleases
/ metabolism
Escherichia coli
/ metabolism
Exoribonucleases
/ metabolism
Exosome Multienzyme Ribonuclease Complex
/ metabolism
Exosomes
/ metabolism
Humans
Protein Subunits
/ metabolism
RNA
/ metabolism
RNA Helicases
/ metabolism
RNA Stability
/ physiology
RNA, Nuclear
/ metabolism
RNA-Binding Proteins
/ metabolism
Recombinant Proteins
/ metabolism
Human
RNA decay
RNA exosome
Ribonuclease
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2020
2020
Historique:
entrez:
27
11
2019
pubmed:
27
11
2019
medline:
15
12
2020
Statut:
ppublish
Résumé
We describe procedures to clone, express, and reconstitute an active human nuclear RNA exosome. Individual recombinant subunits are expressed from E. coli and successfully reconstituted into the nuclear complex, which contains the noncatalytic nine-subunit exosome core, the endoribonuclease and exoribonuclease DIS3, the distributive exoribonuclease EXOSC10, the cofactors C1D and MPP6, and the RNA helicase MTR4.
Identifiants
pubmed: 31768991
doi: 10.1007/978-1-4939-9822-7_23
pmc: PMC8584190
mid: NIHMS1750572
doi:
Substances chimiques
Protein Subunits
0
RNA, Nuclear
0
RNA-Binding Proteins
0
Recombinant Proteins
0
RNA
63231-63-0
Endoribonucleases
EC 3.1.-
Exoribonucleases
EC 3.1.-
Exosome Multienzyme Ribonuclease Complex
EC 3.1.-
RNA Helicases
EC 3.6.4.13
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
467-489Subventions
Organisme : NCI NIH HHS
ID : P30 CA008748
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM079196
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM118080
Pays : United States
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