Construction and evaluation of Vibrio alginolyticus ΔclpP mutant, as a safe live attenuated vibriosis vaccine.

Animals Bacterial Vaccines / administration & dosage Fish Diseases / immunology Mutation Vaccines, Attenuated / administration & dosage Vibrio Infections / immunology Vibrio alginolyticus / genetics
Pearl gentian grouper Vaccine Vibrio alginolyticus Virulence clpP

Journal

Fish & shellfish immunology
ISSN: 1095-9947
Titre abrégé: Fish Shellfish Immunol
Pays: England
ID NLM: 9505220

Informations de publication

Date de publication:
Mar 2020
Historique:
received: 19 08 2019
revised: 08 11 2019
accepted: 22 11 2019
pubmed: 27 11 2019
medline: 18 11 2020
entrez: 27 11 2019
Statut: ppublish

Résumé

Vibrio alginolyticus is a common and serious pathogen threatening the progress of coastal aquaculture. ClpP protease has been proved to be closely associated with biofilm formation, stress tolerance, autolysis and virulence in several pathogens. Hence, targeting ClpP may be a potentially viable, attractive option for the preparation of vaccine in preventing vibriosis. In this study, an in-frame deleted mutant strain (ΔclpP) was constructed by allelic exchange mutagenesis to investigate physiological role of clpP in pathogenicity of V. alginolyticus and evaluate its potential as a live attenuated vaccine. The results exhibited that ΔclpP showed no differences in external morphology, growth, swarming motility and ECPase activity. However, ΔclpP represented an increment in biofilm formation, and a decrement in adherence to CIK cells. In addition, virulence of ΔclpP was examined in pearl gentian grouper and was found to be seriously attenuated. ΔclpP induced high antibody titers and provided a valid protection with a relative percent survival value of 83.8% without histopathologic abnormality. Our results indicated ΔclpP showed a great potential to be a live attenuated vaccine.

Identifiants

DOI: 10.1016/j.fsi.2019.11.054 PMID: 31770644
pubmed: 31770644
pii: S1050-4648(19)31097-6
doi: 10.1016/j.fsi.2019.11.054
pii:
doi:

Substances chimiques

Bacterial Vaccines 0
Vaccines, Attenuated 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

917-922

Informations de copyright

Copyright © 2019 Elsevier Ltd. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of competing interest The authors declare no conflict of interest.

Auteurs

Yanyan Chen (Y)

Fisheries College of Guangdong Ocean University, Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals & Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes, Zhanjiang, China; College of Life Sciences and Technology, Lingnan Normal University, Zhanjiang, China.

Fenglei Wu (F)

Fisheries College of Guangdong Ocean University, Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals & Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes, Zhanjiang, China.

Zhiwen Wang (Z)

Fisheries College of Guangdong Ocean University, Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals & Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes, Zhanjiang, China.

Jufen Tang (J)

Fisheries College of Guangdong Ocean University, Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals & Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes, Zhanjiang, China.

Shuanghu Cai (S)

Fisheries College of Guangdong Ocean University, Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals & Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes, Zhanjiang, China. Electronic address: caish@gdou.edu.cn.

Jichang Jian (J)

Fisheries College of Guangdong Ocean University, Guangdong Provincial Key Laboratory of Pathogenic Biology and Epidemiology for Aquatic Economic Animals & Key Laboratory of Control for Diseases of Aquatic Economic Animals of Guangdong Higher Education Institutes, Zhanjiang, China. Electronic address: jianjc@gdou.edu.cn.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Animaux › Construction and evaluation of Vibrio...
questionsmedicales.fr › Mélanges complexes › Produits biologiques › Vaccins › Vaccins antibactériens › Construction and evaluation of Vibrio...
questionsmedicales.fr › Maladies de l'animal › Maladies des poissons › Construction and evaluation of Vibrio...
questionsmedicales.fr › Phénomènes génétiques › Variation génétique › Mutation › Construction and evaluation of Vibrio...
questionsmedicales.fr › Mélanges complexes › Produits biologiques › Vaccins › Vaccins atténués › Construction and evaluation of Vibrio...
questionsmedicales.fr › Infection › Infections bactériennes et mycoses › Infections bactériennes › Infections bactériennes à Gram négatif › Infections à Vibrio › Construction and evaluation of Vibrio...
questionsmedicales.fr › Bactéries › Proteobacteria › Gammaproteobacteria › Vibrionaceae › Vibrio › Vibrio alginolyticus › Construction and evaluation of Vibrio...