Performance of the Xpert HBV Viral Load assay versus the Aptima Quant assay for quantifying hepatitis B virus DNA.


Journal

Diagnostic microbiology and infectious disease
ISSN: 1879-0070
Titre abrégé: Diagn Microbiol Infect Dis
Pays: United States
ID NLM: 8305899

Informations de publication

Date de publication:
Feb 2020
Historique:
received: 30 07 2019
revised: 07 11 2019
accepted: 13 11 2019
pubmed: 28 11 2019
medline: 10 6 2020
entrez: 28 11 2019
Statut: ppublish

Résumé

Quantification of HBV DNA is used for initiating and monitoring antiviral treatment. We have evaluated the Xpert HBV Viral Load (VL) assay on the GeneXpert instrument. We estimated its limit of detection to be 7.5 IU/mL. Reproducibility was 1.1-12.7% as assessed by the coefficients of variation for 3 different samples. The assay was linear from 2 to 8 log

Identifiants

pubmed: 31771903
pii: S0732-8893(19)30767-9
doi: 10.1016/j.diagmicrobio.2019.114946
pii:
doi:

Substances chimiques

DNA, Viral 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

114946

Informations de copyright

Copyright © 2019 Elsevier Inc. All rights reserved.

Auteurs

Florence Abravanel (F)

INSERM, U1043, Centre de Physiopathologie de Toulouse Purpan, Toulouse, F-31300, France; CHU Toulouse, Hôpital Purpan, Laboratoire de virologie, F-31300, France. Electronic address: abravanel.f@chu-toulouse.fr.

Sébastien Lhomme (S)

INSERM, U1043, Centre de Physiopathologie de Toulouse Purpan, Toulouse, F-31300, France; CHU Toulouse, Hôpital Purpan, Laboratoire de virologie, F-31300, France.

Pauline Trémeaux (P)

CHU Toulouse, Hôpital Purpan, Laboratoire de virologie, F-31300, France.

Marion Migueres (M)

CHU Toulouse, Hôpital Purpan, Laboratoire de virologie, F-31300, France.

Agnès Harter (A)

INSERM, U1043, Centre de Physiopathologie de Toulouse Purpan, Toulouse, F-31300, France.

Catherine Haslé (C)

INSERM, U1043, Centre de Physiopathologie de Toulouse Purpan, Toulouse, F-31300, France.

Patrick Bruel (P)

INSERM, U1043, Centre de Physiopathologie de Toulouse Purpan, Toulouse, F-31300, France.

Laurent Alric (L)

CHU Toulouse, Hôpital Purpan, Fédération digestive, F-31300, France.

Sophie Métivier (S)

CHU Toulouse, Hôpital Purpan, Fédération digestive, F-31300, France.

Stéphanie Raymond (S)

INSERM, U1043, Centre de Physiopathologie de Toulouse Purpan, Toulouse, F-31300, France; CHU Toulouse, Hôpital Purpan, Laboratoire de virologie, F-31300, France.

Jacques Izopet (J)

INSERM, U1043, Centre de Physiopathologie de Toulouse Purpan, Toulouse, F-31300, France; CHU Toulouse, Hôpital Purpan, Laboratoire de virologie, F-31300, France.

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Classifications MeSH