Neonatal Wnt-dependent Lgr5 positive stem cells are essential for uterine gland development.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
26 11 2019
26 11 2019
Historique:
received:
21
12
2018
accepted:
05
11
2019
entrez:
28
11
2019
pubmed:
28
11
2019
medline:
14
4
2020
Statut:
epublish
Résumé
Wnt signaling is critical for directing epithelial gland development within the uterine lining to ensure successful gestation in adults. Wnt-dependent, Lgr5-expressing stem/progenitor cells are essential for the development of glandular epithelia in the intestine and stomach, but their existence in the developing reproductive tract has not been investigated. Here, we employ Lgr5-2A-EGFP/CreERT2/DTR mouse models to identify Lgr5-expressing cells in the developing uterus and to evaluate their stem cell identity and function. Lgr5 is broadly expressed in the uterine epithelium during embryogenesis, but becomes largely restricted to the tips of developing glands after birth. In-vivo lineage tracing/ablation/organoid culture assays identify these gland-resident Lgr5
Identifiants
pubmed: 31772170
doi: 10.1038/s41467-019-13363-3
pii: 10.1038/s41467-019-13363-3
pmc: PMC6879518
doi:
Substances chimiques
Lgr5 protein, mouse
0
Receptors, G-Protein-Coupled
0
Wnt Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
5378Références
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