Metabolic Labeling of Inositol Phosphates and Phosphatidylinositols in Yeast and Mammalian Cells.
HPLC
Inositol phosphates
Lipid signaling
Metabolic labeling
Phosphatidylinositol
Signal transduction
Journal
Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969
Informations de publication
Date de publication:
2020
2020
Historique:
entrez:
28
11
2019
pubmed:
28
11
2019
medline:
20
1
2021
Statut:
ppublish
Résumé
Inositol phosphate (IP) and phosphatidylinositol (PI) signaling are critical signal transduction pathways responsible for generating numerous receptor-mediated cellular responses. Biochemical and genetic studies have revealed diverse roles of IP and PI signaling in eukaryotic signaling, but detailed characterization of unique IP and PI signaling profiles in response to different agonists and among cell types remains largely unexplored. Here, we outline steady-state inositol metabolic-labeling techniques that can be leveraged to assess the IP and PI signaling state in eukaryotic cells. This flexible technique can be amended and optimized to your cell line of interest, perturbed with biochemical, genetic, or pharmacological alteration, and used to provide comprehensive inositol profiling in various cellular systems.
Identifiants
pubmed: 31773572
doi: 10.1007/978-1-0716-0167-9_7
pmc: PMC7293352
mid: NIHMS1586438
doi:
Substances chimiques
Inositol Phosphates
0
Phosphatidylinositols
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
83-92Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM124404
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL055672
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM007347
Pays : United States
Références
Prog Lipid Res. 2010 Jul;49(3):201-17
pubmed: 20043944
Subcell Biochem. 2012;58:215-79
pubmed: 22403078
Mol Cell Biol. 2010 Sep;30(18):4535-47
pubmed: 20624911
Trends Cell Biol. 2013 Jun;23(6):270-8
pubmed: 23489878
Annu Rev Biochem. 1992;61:225-50
pubmed: 1323235
J Biol Chem. 2005 Jan 14;280(2):1156-64
pubmed: 15528195
Methods Mol Biol. 2010;645:47-60
pubmed: 20645180
Biochim Biophys Acta. 2015 May;1851(5):620-8
pubmed: 25633344
Nature. 1990 Aug 9;346(6284):580-3
pubmed: 2198472
Methods Enzymol. 2007;434:171-85
pubmed: 17954248
Sci Signal. 2012 Jun 19;5(229):ra44
pubmed: 22715467
Sci Signal. 2011 Aug 23;4(188):re1
pubmed: 21878680
J Biol Chem. 2002 Nov 8;277(45):42711-8
pubmed: 12226109
Nature. 1989 Sep 21;341(6239):197-205
pubmed: 2550825
Adv Enzyme Regul. 2010;50(1):324-37
pubmed: 20006638
Proc Natl Acad Sci U S A. 2007 Oct 2;104(40):15653-8
pubmed: 17895383
Cell Mol Life Sci. 2006 Mar;63(5):552-64
pubmed: 16429326
Science. 2007 Apr 6;316(5821):109-12
pubmed: 17412959
J Lipid Res. 2009 Apr;50 Suppl:S249-54
pubmed: 19001665
Trends Biochem Sci. 2012 Apr;37(4):134-43
pubmed: 22381590
Methods. 2006 Jun;39(2):112-21
pubmed: 16829132
Science. 2002 May 31;296(5573):1655-7
pubmed: 12040186
Proc Natl Acad Sci U S A. 2005 Jun 14;102(24):8454-9
pubmed: 15939867
J Biol Chem. 2004 Nov 5;279(45):47222-32
pubmed: 15322119
J Biol Chem. 2005 Jul 29;280(30):27654-61
pubmed: 15944147
Cell. 2010 Dec 10;143(6):1030-1030.e1
pubmed: 21145466
Cell. 2017 Aug 10;170(4):605-635
pubmed: 28802037
Dev Cell. 2009 Jan;16(1):12-20
pubmed: 19154715
Nat Rev Mol Cell Biol. 2012 Feb 23;13(3):195-203
pubmed: 22358332
Biochem Soc Trans. 2016 Feb;44(1):240-52
pubmed: 26862211
Biochem J. 2014 Jul 15;461(2):159-75
pubmed: 24966051
J Biol Chem. 2005 Feb 11;280(6):4264-9
pubmed: 15561716