Freezing and storage effects on enzyme, electrolyte, and mineral compositions in canine prostatic fluid.
dog
enzyme activity
minerals
seminal plasma
Journal
Veterinary clinical pathology
ISSN: 1939-165X
Titre abrégé: Vet Clin Pathol
Pays: United States
ID NLM: 9880575
Informations de publication
Date de publication:
Dec 2019
Dec 2019
Historique:
received:
05
11
2018
revised:
24
02
2019
accepted:
22
04
2019
pubmed:
28
11
2019
medline:
3
6
2020
entrez:
28
11
2019
Statut:
ppublish
Résumé
There is a paucity of information about the storage stability of seminal plasma components in literature. This study investigates the potential effects of storage at -18.0°C on the enzyme activities and concentrations of selected electrolytes and minerals in canine prostatic fluid. Semen was obtained in fractions from ten male dogs, and the third ejaculate fraction was analyzed immediately after sampling and after storage at -18.0°C for 7 days. The following analytes were assessed: gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), sodium, potassium, total calcium, inorganic phosphate, magnesium, zinc, and copper. Whereas electrolyte and mineral concentrations were not affected by freezing and 7 days of storage, effects on the enzyme activities were variable. ALP, AST, and LDH activities were significantly decreased (P < .05) in the post-thaw samples compared with the freshly prepared samples. Because freezing and storage of seminal plasma at -18°C had an impact on the activity of some enzymes, analyses should be performed in fresh, not frozen samples.
Sections du résumé
BACKGROUND
BACKGROUND
There is a paucity of information about the storage stability of seminal plasma components in literature.
OBJECTIVES
OBJECTIVE
This study investigates the potential effects of storage at -18.0°C on the enzyme activities and concentrations of selected electrolytes and minerals in canine prostatic fluid.
METHODS
METHODS
Semen was obtained in fractions from ten male dogs, and the third ejaculate fraction was analyzed immediately after sampling and after storage at -18.0°C for 7 days. The following analytes were assessed: gamma-glutamyl transferase (GGT), alkaline phosphatase (ALP), aspartate aminotransferase (AST), lactate dehydrogenase (LDH), sodium, potassium, total calcium, inorganic phosphate, magnesium, zinc, and copper.
RESULTS
RESULTS
Whereas electrolyte and mineral concentrations were not affected by freezing and 7 days of storage, effects on the enzyme activities were variable. ALP, AST, and LDH activities were significantly decreased (P < .05) in the post-thaw samples compared with the freshly prepared samples.
CONCLUSIONS
CONCLUSIONS
Because freezing and storage of seminal plasma at -18°C had an impact on the activity of some enzymes, analyses should be performed in fresh, not frozen samples.
Substances chimiques
Electrolytes
0
Minerals
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
691-694Informations de copyright
© 2019 American Society for Veterinary Clinical Pathology.
Références
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