Tissue traction microscopy to quantify muscle contraction within precision-cut lung slices.
ASM
airway
contraction
stretch
Journal
American journal of physiology. Lung cellular and molecular physiology
ISSN: 1522-1504
Titre abrégé: Am J Physiol Lung Cell Mol Physiol
Pays: United States
ID NLM: 100901229
Informations de publication
Date de publication:
01 02 2020
01 02 2020
Historique:
pubmed:
28
11
2019
medline:
13
5
2020
entrez:
28
11
2019
Statut:
ppublish
Résumé
In asthma, acute bronchospasm is driven by contractile forces of airway smooth muscle (ASM). These forces can be imaged in the cultured ASM cell or assessed in the muscle strip and the tracheal/bronchial ring, but in each case, the ASM is studied in isolation from the native airway milieu. Here, we introduce a novel platform called tissue traction microscopy (TTM) to measure ASM contractile force within porcine and human precision-cut lung slices (PCLS). Compared with the conventional measurements of lumen area changes in PCLS, TTM measurements of ASM force changes are
Identifiants
pubmed: 31774304
doi: 10.1152/ajplung.00297.2019
pmc: PMC7052683
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
L323-L330Subventions
Organisme : NHLBI NIH HHS
ID : P01 HL091842
Pays : United States
Organisme : NHLBI NIH HHS
ID : K08 HL135443
Pays : United States
Organisme : NHLBI NIH HHS
ID : R21 HL123522
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM007337
Pays : United States
Organisme : NHLBI NIH HHS
ID : UH3 HL123816
Pays : United States
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