Cell-Type-Specific Complement Expression in the Healthy and Diseased Retina.
Müller cell
age-dependent
alternative
classical
complement expression
eye
ischemia
lectin
neuron
retina
Journal
Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691
Informations de publication
Date de publication:
26 11 2019
26 11 2019
Historique:
received:
11
01
2019
revised:
24
05
2019
accepted:
22
10
2019
entrez:
28
11
2019
pubmed:
28
11
2019
medline:
21
10
2020
Statut:
ppublish
Résumé
Complement dysregulation is a feature of many retinal diseases, yet mechanistic understanding at the cellular level is limited. Given this knowledge gap about which retinal cells express complement, we performed single-cell RNA sequencing on ∼92,000 mouse retinal cells and validated our results in five major purified retinal cell types. We found evidence for a distributed cell-type-specific complement expression across 11 cell types. Notably, Müller cells are the major contributor of complement activators c1s, c3, c4, and cfb. Retinal pigment epithelium (RPE) mainly expresses cfh and the terminal complement components, whereas cfi and cfp transcripts are most abundant in neurons. Aging enhances c1s, cfb, cfp, and cfi expression, while cfh expression decreases. Transient retinal ischemia increases complement expression in microglia, Müller cells, and RPE. In summary, we report a unique complement expression signature for murine retinal cell types suggesting a well-orchestrated regulation of local complement expression in the retinal microenvironment.
Identifiants
pubmed: 31775049
pii: S2211-1247(19)31406-8
doi: 10.1016/j.celrep.2019.10.084
pmc: PMC6911814
mid: NIHMS1544713
pii:
doi:
Substances chimiques
Complement System Proteins
9007-36-7
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2835-2848.e4Subventions
Organisme : NEI NIH HHS
ID : R01 EY030192
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM108600
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM125301
Pays : United States
Organisme : NHGRI NIH HHS
ID : R01 HG006137
Pays : United States
Informations de copyright
Copyright © 2019 The Author(s). Published by Elsevier Inc. All rights reserved.
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