Development and comparison of enzyme-linked immunosorbent assays based on recombinant trimeric full-length and truncated spike proteins for detecting antibodies against porcine epidemic diarrhea virus.
Human embryonic kidney (HEK)-293 mammalian cell expression system
Indirect ELISA
Porcine epidemic diarrhea (PED)
Serology
Spike protein specific ELISA
Journal
BMC veterinary research
ISSN: 1746-6148
Titre abrégé: BMC Vet Res
Pays: England
ID NLM: 101249759
Informations de publication
Date de publication:
27 Nov 2019
27 Nov 2019
Historique:
received:
01
03
2019
accepted:
08
11
2019
entrez:
29
11
2019
pubmed:
30
11
2019
medline:
24
3
2020
Statut:
epublish
Résumé
Since 2010, outbreaks of genotype 2 (G2) porcine epidemic diarrhea virus (PEDV) have caused high mortality in neonatal piglets and have had devastating impacts on the swine industry in many countries. A reliable serological assay for evaluating the PEDV-specific humoral and mucosal immune response is important for disease survey, monitoring the efficacy of immunization, and designing strategies for the prevention and control of PED. Two PEDV spike (S) glycoprotein-based indirect enzyme-linked immunosorbent assays (ELISAs) were developed using G2b PEDV-Pintung 52 (PEDV-PT) trimeric full-length S and truncated S The commercial N-based ELISA exhibited a sensitivity of 37%, a specificity of 100%, and a fair agreement (kappa = 0.37) with the immunostaining result. In comparison, the full-length S-based ELISA showed a sensitivity of 97.8%, a specificity of 94%, and an almost perfect agreement (kappa = 0.90) with the immunostaining result. Interestingly, the S Both full-length S-based and S
Sections du résumé
BACKGROUND
BACKGROUND
Since 2010, outbreaks of genotype 2 (G2) porcine epidemic diarrhea virus (PEDV) have caused high mortality in neonatal piglets and have had devastating impacts on the swine industry in many countries. A reliable serological assay for evaluating the PEDV-specific humoral and mucosal immune response is important for disease survey, monitoring the efficacy of immunization, and designing strategies for the prevention and control of PED. Two PEDV spike (S) glycoprotein-based indirect enzyme-linked immunosorbent assays (ELISAs) were developed using G2b PEDV-Pintung 52 (PEDV-PT) trimeric full-length S and truncated S
RESULTS
RESULTS
The commercial N-based ELISA exhibited a sensitivity of 37%, a specificity of 100%, and a fair agreement (kappa = 0.37) with the immunostaining result. In comparison, the full-length S-based ELISA showed a sensitivity of 97.8%, a specificity of 94%, and an almost perfect agreement (kappa = 0.90) with the immunostaining result. Interestingly, the S
CONCLUSIONS
CONCLUSIONS
Both full-length S-based and S
Identifiants
pubmed: 31775769
doi: 10.1186/s12917-019-2171-7
pii: 10.1186/s12917-019-2171-7
pmc: PMC6880432
doi:
Substances chimiques
Antibodies, Viral
0
Spike Glycoprotein, Coronavirus
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
421Références
Virus Res. 2016 Dec 2;226:117-127
pubmed: 27317167
J Clin Microbiol. 2017 May;55(5):1426-1436
pubmed: 28202790
Clin Exp Vaccine Res. 2015 Jul;4(2):166-76
pubmed: 26273575
Adv Virus Res. 1997;48:1-100
pubmed: 9233431
Transbound Emerg Dis. 2019 Jan;66(1):111-118
pubmed: 30102851
J Virol. 2008 Jun;82(12):6078-83
pubmed: 18400867
BMC Vet Res. 2018 Mar 14;14(1):96
pubmed: 29540176
Transbound Emerg Dis. 2018 Jun;65(3):883-889
pubmed: 29388343
Curr Opin Virol. 2019 Feb;34:39-49
pubmed: 30654269
Emerg Infect Dis. 2012 Jan;18(1):161-3
pubmed: 22261231
Annu Rev Biochem. 2011;80:71-99
pubmed: 21495850
J Virol. 2017 May 26;91(12):
pubmed: 28381581
Methods Mol Biol. 2015;1261:115-27
pubmed: 25502196
Virology. 2017 Sep;509:185-194
pubmed: 28647506
Viruses. 2012 Jun;4(6):1011-33
pubmed: 22816037
J Virol Methods. 2015 Dec 1;225:90-4
pubmed: 26253335
Biotechnol Lett. 2011 Feb;33(2):215-20
pubmed: 20882317
Vet Microbiol. 2000 Mar 15;72(3-4):173-82
pubmed: 10727829
J Virol. 2015 Mar;89(6):3332-42
pubmed: 25589635
Virus Genes. 1995;10(2):137-48
pubmed: 8560773
Vet Microbiol. 1993 Nov;37(3-4):285-97
pubmed: 8116187
Vet Microbiol. 2007 Jul 20;123(1-3):86-92
pubmed: 17368968
J Gen Virol. 2005 Sep;86(Pt 9):2543-52
pubmed: 16099913
BMC Vet Res. 2015 Aug 01;11:180
pubmed: 26232106
BMC Vet Res. 2018 Aug 20;14(1):243
pubmed: 30126390
Nature. 2016 Mar 3;531(7592):114-117
pubmed: 26855426
Vet Microbiol. 2015 Jul 9;178(1-2):31-40
pubmed: 25939885
Arch Virol. 1978;58(3):243-7
pubmed: 83132
PLoS Pathog. 2013 Sep;9(9):e1003618
pubmed: 24068931
Acta Virol. 2007;51(3):149-56
pubmed: 18076304
Viruses. 2017 May 19;9(5):
pubmed: 28534849
Vet J. 2014 Oct;202(1):33-6
pubmed: 25135339
Viruses. 2018 Jun 27;10(7):
pubmed: 29954081
Arch Virol. 2014 Nov;159(11):2977-87
pubmed: 25008896
Vet J. 2015 May;204(2):134-43
pubmed: 25841898
Transbound Emerg Dis. 2018 Jun;65(3):660-675
pubmed: 29392870
Appl Microbiol Biotechnol. 2018 Sep;102(17):7499-7507
pubmed: 29961099
Vet Q. 2014;34(4):218-23
pubmed: 25415042
Virus Res. 2016 Dec 2;226:85-92
pubmed: 27287711
Vet Microbiol. 1992 Sep;32(2):117-34
pubmed: 1441196
Mol Cells. 2002 Oct 31;14(2):295-9
pubmed: 12442904
Nat Rev Mol Cell Biol. 2012 Jun 22;13(7):448-62
pubmed: 22722607
Virus Res. 2008 Mar;132(1-2):192-6
pubmed: 18067984
Sci Rep. 2019 Feb 21;9(1):2529
pubmed: 30792462