Nucleocapsid Protein Recruitment to Replication-Transcription Complexes Plays a Crucial Role in Coronaviral Life Cycle.
coronavirus
nucleocapsid N protein
replication-transcription complexes
viral mRNA synthesis
Journal
Journal of virology
ISSN: 1098-5514
Titre abrégé: J Virol
Pays: United States
ID NLM: 0113724
Informations de publication
Date de publication:
31 01 2020
31 01 2020
Historique:
received:
15
11
2019
accepted:
17
11
2019
pubmed:
30
11
2019
medline:
26
6
2020
entrez:
29
11
2019
Statut:
epublish
Résumé
Coronavirus (CoV) nucleocapsid (N) proteins are key for incorporating genomic RNA into progeny viral particles. In infected cells, N proteins are present at the replication-transcription complexes (RTCs), the sites of CoV RNA synthesis. It has been shown that N proteins are important for viral replication and that the one of mouse hepatitis virus (MHV), a commonly used model CoV, interacts with nonstructural protein 3 (nsp3), a component of the RTCs. These two aspects of the CoV life cycle, however, have not been linked. We found that the MHV N protein binds exclusively to nsp3 and not other RTC components by using a systematic yeast two-hybrid approach, and we identified two distinct regions in the N protein that redundantly mediate this interaction. A selective N protein variant carrying point mutations in these two regions fails to bind nsp3
Identifiants
pubmed: 31776274
pii: JVI.01925-19
doi: 10.1128/JVI.01925-19
pmc: PMC6997762
pii:
doi:
Substances chimiques
Nucleocapsid Proteins
0
RNA, Viral
0
Viral Nonstructural Proteins
0
nucleocapsid protein, Hepatitis virus
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
Copyright © 2020 American Society for Microbiology.
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