Boosting with AIDSVAX B/E Enhances Env Constant Region 1 and 2 Antibody-Dependent Cellular Cytotoxicity Breadth and Potency.


Journal

Journal of virology
ISSN: 1098-5514
Titre abrégé: J Virol
Pays: United States
ID NLM: 0113724

Informations de publication

Date de publication:
31 01 2020
Historique:
received: 11 07 2019
accepted: 25 10 2019
pubmed: 30 11 2019
medline: 22 8 2020
entrez: 29 11 2019
Statut: epublish

Résumé

Induction of protective antibodies is a critical goal of HIV-1 vaccine development. One strategy is to induce nonneutralizing antibodies (NNAbs) that kill virus-infected cells, as these antibody specificities have been implicated in slowing HIV-1 disease progression and in protection. HIV-1 Env constant region 1 and 2 (C1C2) monoclonal antibodies (MAbs) frequently mediate potent antibody-dependent cellular cytotoxicity (ADCC), making them an important vaccine target. Here, we explore the effect of delayed and repetitive boosting of RV144 vaccine recipients with AIDSVAX B/E on the C1C2-specific MAb repertoire. It was found that boosting increased clonal lineage-specific ADCC breadth and potency. A ligand crystal structure of a vaccine-induced broad and potent ADCC-mediating C1C2-specific MAb showed that it bound a highly conserved Env gp120 epitope. Thus, boosting to affinity mature these types of IgG C1C2-specific antibody responses may be one method by which to make an improved HIV vaccine with higher efficacy than that seen in the RV144 trial.

Identifiants

pubmed: 31776278
pii: JVI.01120-19
doi: 10.1128/JVI.01120-19
pmc: PMC6997759
pii:
doi:

Substances chimiques

AIDS Vaccines 0
AIDSVAX 0
AIDSVAX B-E 0
Antibodies, Monoclonal 0
Epitopes 0
HIV Antibodies 0
HIV Envelope Protein gp120 0
Immunoglobulin G 0

Types de publication

Journal Article Randomized Controlled Trial Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : NIAID NIH HHS
ID : R01 AI129769
Pays : United States
Organisme : NIAMS NIH HHS
ID : T32 AR007592
Pays : United States
Organisme : NIH HHS
ID : K01 OD024877
Pays : United States
Organisme : NIAID NIH HHS
ID : P01 AI120756
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA014236
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI116274
Pays : United States

Informations de copyright

Copyright © 2020 Easterhoff et al.

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Auteurs

David Easterhoff (D)

Duke University, Durham, North Carolina, USA david.easterhoff@duke.edu guido.ferrari@duke.edu.

Justin Pollara (J)

Duke University, Durham, North Carolina, USA.

Kan Luo (K)

Duke University, Durham, North Carolina, USA.

William D Tolbert (WD)

Infectious Diseases Division, Uniformed Services University of the Health Sciences, Bethesda, Maryland, USA.

Brianna Young (B)

Department of Biochemistry and Molecular Biology, University of Maryland, Baltimore, Maryland, USA.

Dieter Mielke (D)

Duke University, Durham, North Carolina, USA.

Shalini Jha (S)

Duke University, Durham, North Carolina, USA.

Robert J O'Connell (RJ)

U.S. Army Medical Directorate, AFRIMS, Bangkok, Thailand.

Sandhya Vasan (S)

U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
U.S. Army Medical Directorate, AFRIMS, Bangkok, Thailand.
The Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, Maryland, USA.

Jerome Kim (J)

U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.

Nelson L Michael (NL)

U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
The Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, Maryland, USA.

Jean-Louis Excler (JL)

U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
The Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, Maryland, USA.

Merlin L Robb (ML)

U.S. Military HIV Research Program, Walter Reed Army Institute of Research, Silver Spring, Maryland, USA.
The Henry M. Jackson Foundation for the Advancement of Military Medicine, Bethesda, Maryland, USA.

Supachai Rerks-Ngarm (S)

Thai Ministry of Public Health, Nonthaburi, Thailand.

Jaranit Kaewkungwal (J)

Mahidol University, Bangkok, Thailand.

Punnee Pitisuttithum (P)

Mahidol University, Bangkok, Thailand.

Sorachai Nitayaphan (S)

Royal Thai Army Component, AFRIMS, Bangkok, Thailand.

Faruk Sinangil (F)

Global Solutions of Infectious Diseases, South San Francisco, California, USA.

James Tartaglia (J)

Sanofi Pasteur, Swiftwater, Pennsylvania, USA.

Sanjay Phogat (S)

Sanofi Pasteur, Swiftwater, Pennsylvania, USA.

Thomas B Kepler (TB)

Boston University, Boston, Massachusetts, USA.

S Munir Alam (SM)

Duke University, Durham, North Carolina, USA.

Kevin Wiehe (K)

Duke University, Durham, North Carolina, USA.

Kevin O Saunders (KO)

Duke University, Durham, North Carolina, USA.

David C Montefiori (DC)

Duke University, Durham, North Carolina, USA.

Georgia D Tomaras (GD)

Duke University, Durham, North Carolina, USA.

M Anthony Moody (MA)

Duke University, Durham, North Carolina, USA.

Marzena Pazgier (M)

Infectious Diseases Division, Uniformed Services University of the Health Sciences, Bethesda, Maryland, USA.

Barton F Haynes (BF)

Duke University, Durham, North Carolina, USA.

Guido Ferrari (G)

Duke University, Durham, North Carolina, USA david.easterhoff@duke.edu guido.ferrari@duke.edu.

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