Profiling of hydroxyurea-treated β-thalassemia/ serum proteome through nano-LC-ESI-MS/ MS in combination with microsol-isoelectric focusing.
Biomarkers
/ analysis
Blood Proteins
/ analysis
Chromatography, Liquid
/ methods
Humans
Hydroxyurea
/ chemistry
Isoelectric Focusing
/ methods
Nanomedicine
Proteome
/ analysis
Proteomics
/ methods
Spectrometry, Mass, Electrospray Ionization
/ methods
Tandem Mass Spectrometry
/ methods
beta-Thalassemia
/ blood
biomarkers
hydroxyurea
microsolution isoelectric focusing
nano-LC-MS/ MS
one-dimensional gel electrophoresis
protein fractionation
proteomics
β-thalassemia
Journal
Biomedical chromatography : BMC
ISSN: 1099-0801
Titre abrégé: Biomed Chromatogr
Pays: England
ID NLM: 8610241
Informations de publication
Date de publication:
Mar 2020
Mar 2020
Historique:
received:
21
05
2019
revised:
30
09
2019
accepted:
08
11
2019
pubmed:
30
11
2019
medline:
1
4
2020
entrez:
29
11
2019
Statut:
ppublish
Résumé
Advancements in proteomic tools offer a comprehensive solution to studying the complexity of diseases at molecular level. This study focusses on the clinical proteomic profiling of pre- and post-hydroxyurea (HU)-treated β-thalassemia patients in parallel with healthy individuals to better understand the role of HU in the treatment of β-thalassemia. The strategy encompasses sequential high-resolution protein fractionation using MicroSol-isoelectric focusing (ZOOM- IEF) followed by one-dimensional SDS-PAGE before nano-RP-LC-MS/ MS analysis of tryptic peptides. Protein identification was performed through Mascot search using NCBInr and SwissProt databases. Several different proteins were observed in pool serum samples of each of the three study groups. Approximately, 1250 proteins exclusive to each group were identified, and after removing the redundant and low sequence coverage proteins, the number was reduced to 576 (201 in healthy, 187 in HU-untreated and 188 in HU-treated group). Uniquely identified proteins in the HU-treated group regulate the focal adhesion, ECM-receptor interaction, PI3K-Akt signaling, Rap1 signaling, cAMP signaling, platelet activation, and Ca
Substances chimiques
Biomarkers
0
Blood Proteins
0
Proteome
0
Hydroxyurea
X6Q56QN5QC
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e4753Informations de copyright
© 2019 John Wiley & Sons, Ltd.
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