Late-stage difluoromethylation leading to a self-immobilizing fluorogenic probe for the visualization of enzyme activities in live cells.

Fluorescent Dyes / metabolism HEK293 Cells HeLa Cells Humans Indolequinones / metabolism Methylation Spectrometry, Fluorescence beta-Galactosidase / metabolism

Journal

Chemical communications (Cambridge, England)
ISSN: 1364-548X
Titre abrégé: Chem Commun (Camb)
Pays: England
ID NLM: 9610838

Informations de publication

Date de publication:
10 Dec 2019
Historique:
pubmed: 30 11 2019
medline: 1 1 2020
entrez: 29 11 2019
Statut: ppublish

Résumé

Reported herein is a novel p-quinone methide-based self-immobilizing fluorogenic probe for the visualization of β-galactosidase activities in live cells. This easily prepared imaging reagent massively increases the fluorescence intensity and covalently links to the activation site with high efficiency upon enzymatic trigger.

Identifiants

DOI: 10.1039/c9cc07903c PMID: 31777880
pubmed: 31777880
doi: 10.1039/c9cc07903c
doi:

Substances chimiques

Fluorescent Dyes 0
Indolequinones 0
quinone methide 138230-21-4
beta-Galactosidase EC 3.2.1.23

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

15000-15003

Auteurs

Jialing Jiang (J)

State Key Laboratory of Bioreactor Engineering, Shanghai Key Laboratory of New Drug Design, School of Pharmacy, East China University of Science and Technology, Shanghai 200237, China. xiehexin@ecust.edu.cn.

Qingwei Tan (Q)

Shuxuan Zhao (S)

Heng Song (H)

Liqiang Hu (L)

Hexin Xie (H)

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Classifications MeSH

questionsmedicales.fr › Actions chimiques et utilisations › Utilisations spécialisées de produits chimiques › Produits chimiques de laboratoire › Indicateurs et réactifs › Luminescents › Colorants fluorescents › Late-stage difluoromethylation leading to a...
questionsmedicales.fr › Cellules › Cellules épithéliales › Cellules HEK293 › Late-stage difluoromethylation leading to a...
questionsmedicales.fr › Cellules › Cellules épithéliales › Cellules HeLa › Late-stage difluoromethylation leading to a...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Late-stage difluoromethylation leading to a...
questionsmedicales.fr › Composés hétérocycliques › Composés hétérocycliques à cycles fusionnés › Composés hétérobicycliques › Indoles › Indolequinones › Late-stage difluoromethylation leading to a...
questionsmedicales.fr › Métabolisme › Alkylation › Méthylation › Late-stage difluoromethylation leading to a...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Analyse spectrale › Spectrométrie de fluorescence › Late-stage difluoromethylation leading to a...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Hydrolases › Glycosidases › Galactosidases › beta-Galactosidase › Late-stage difluoromethylation leading to a...