Phenotypic traits of mesenchymal stem cell sheets fabricated by temperature-responsive cell culture plate: structural characteristics of MSC sheets.

Cell Adhesion Cell Culture Techniques Extracellular Matrix / metabolism Humans Intercellular Junctions / metabolism Mesenchymal Stem Cells / cytology

Journal

Stem cell research & therapy
ISSN: 1757-6512
Titre abrégé: Stem Cell Res Ther
Pays: England
ID NLM: 101527581

Informations de publication

Date de publication:
28 11 2019
Historique:
received: 28 06 2019
accepted: 30 09 2019
revised: 15 09 2019
entrez: 30 11 2019
pubmed: 30 11 2019
medline: 22 7 2020
Statut: epublish

Résumé

In most stem cell therapy strategies reported to date, stem cells are introduced to damaged tissue sites to repair and regenerate the original tissue structure and function. MSC therapeutic efficacies are inconsistent, largely attributed to transplanted MSC difficulties both in engrafting at tissue sites and in retaining their therapeutic functions from suspension formulations. MSC functional components, including cell adhesion and cell-cell junction proteins, and ECM that contribute to essential cellular therapeutic effects, are damaged or removed by proteolytic enzymes used in stem cell harvesting strategies from culture. To overcome these limitations, methods to harvest and transplant cells without disrupting critical stem cell functions are required. Cell sheet technology, exploiting temperature-responsive cell culture surfaces, permits cell harvest without cell protein damage. This study is focused on phenotypic traits of MSC sheets structurally and functionally to understand therapeutic benefits of cell sheets. This study verified cleaved cellular proteins (vinculin, fibronectin, laminin, integrin β-1, and connexin 43) and increased apoptotic cell death produced under standard trypsin harvesting treatment in a time-dependent manner. However, MSC sheets produced without trypsin using only temperature-controlled sheet harvest from culture plastic exhibited intact cellular structures. Also, MSCs harvested using enzymatic treatment (i.e., chemical disruption) showed higher pYAP expression compared to MSC sheets. Retention of cellular structures such as ECM, cell-cell junctions, and cell-ECM junctions is correlated with human umbilical cord mesenchymal stem cell (hUC-MSC) survival after detachment from cell culture surfaces. Retaining these proteins intact in MSC cultures using cell sheet technology is proposed to enhance stem cell survival and their function in stem cell-based therapy.

Sections du résumé

BACKGROUND
In most stem cell therapy strategies reported to date, stem cells are introduced to damaged tissue sites to repair and regenerate the original tissue structure and function. MSC therapeutic efficacies are inconsistent, largely attributed to transplanted MSC difficulties both in engrafting at tissue sites and in retaining their therapeutic functions from suspension formulations. MSC functional components, including cell adhesion and cell-cell junction proteins, and ECM that contribute to essential cellular therapeutic effects, are damaged or removed by proteolytic enzymes used in stem cell harvesting strategies from culture. To overcome these limitations, methods to harvest and transplant cells without disrupting critical stem cell functions are required. Cell sheet technology, exploiting temperature-responsive cell culture surfaces, permits cell harvest without cell protein damage. This study is focused on phenotypic traits of MSC sheets structurally and functionally to understand therapeutic benefits of cell sheets.
METHODS/RESULTS
This study verified cleaved cellular proteins (vinculin, fibronectin, laminin, integrin β-1, and connexin 43) and increased apoptotic cell death produced under standard trypsin harvesting treatment in a time-dependent manner. However, MSC sheets produced without trypsin using only temperature-controlled sheet harvest from culture plastic exhibited intact cellular structures. Also, MSCs harvested using enzymatic treatment (i.e., chemical disruption) showed higher pYAP expression compared to MSC sheets.
CONCLUSION
Retention of cellular structures such as ECM, cell-cell junctions, and cell-ECM junctions is correlated with human umbilical cord mesenchymal stem cell (hUC-MSC) survival after detachment from cell culture surfaces. Retaining these proteins intact in MSC cultures using cell sheet technology is proposed to enhance stem cell survival and their function in stem cell-based therapy.

Identifiants

DOI: 10.1186/s13287-019-1431-6 PMID: 31779694 PMC: PMC6883536
pubmed: 31779694
doi: 10.1186/s13287-019-1431-6
pii: 10.1186/s13287-019-1431-6
pmc: PMC6883536
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

353

Références

Biomaterials. 2006 Sep;27(27):4765-74
pubmed: 16737736
NPJ Regen Med. 2017 Mar 24;2:6
pubmed: 29302343
Curr Opin Cell Biol. 2012 Oct;24(5):600-6
pubmed: 22980731
Cell Biol Int. 2010 May 07;34(6):663-8
pubmed: 20337597
Surg Today. 2012 Jan;42(2):181-4
pubmed: 22200756
Cell. 2012 Aug 17;150(4):669-70
pubmed: 22901800
Biomaterials. 2012 May;33(15):3846-51
pubmed: 22369960
Sci Rep. 2018 Jan 10;8(1):360
pubmed: 29321630
Langmuir. 2004 Jun 22;20(13):5506-11
pubmed: 15986693
N Engl J Med. 2017 Mar 16;376(11):1005-1007
pubmed: 28146644
Transfusion. 2007 Aug;47(8):1426-35
pubmed: 17655587
Trends Mol Med. 2013 Sep;19(9):555-64
pubmed: 23790684
Cell Signal. 2014 Feb;26(2):186-91
pubmed: 24216612
Diabetes. 2015 Aug;64(8):2723-34
pubmed: 25795216
Nat Protoc. 2015 Dec;10(12):2074-80
pubmed: 26584447
N Engl J Med. 2004 Sep 16;351(12):1187-96
pubmed: 15371576
NPJ Regen Med. 2017 Oct 11;2:26
pubmed: 29302360
Biomaterials. 2007 Sep;28(27):3928-35
pubmed: 17570483
Cell Transplant. 2013;22(3):513-9
pubmed: 23057960
Biol Proced Online. 2009 Mar 10;11:161-9
pubmed: 19495917
Cytotherapy. 2013 Apr;15(4):416-22
pubmed: 23480951
Cold Spring Harb Perspect Biol. 2017 Jul 5;9(7):null
pubmed: 28679638
Sci Signal. 2010 Oct 26;3(145):re8
pubmed: 20978240
J Transl Med. 2018 Mar 9;16(1):57
pubmed: 29523171
Nat Med. 2006 Apr;12(4):459-65
pubmed: 16582917
Transpl Int. 2007 Sep;20(9):731-46
pubmed: 17555531
Tissue Eng Part A. 2011 Dec;17(23-24):2973-80
pubmed: 21875331
Annu Rev Biomed Eng. 2012;14:325-49
pubmed: 22809140
Cell Stem Cell. 2008 Mar 6;2(3):205-13
pubmed: 18371446
Genes Dev. 2012 Jan 1;26(1):54-68
pubmed: 22215811
Int Endod J. 2007 May;40(5):338-43
pubmed: 17403041
Biomed Res Int. 2014;2014:951512
pubmed: 24511552
J Endocrinol. 2011 May;209(2):139-51
pubmed: 21307119
Cell Death Differ. 2006 Mar;13(3):363-73
pubmed: 16397583
Stem Cells Int. 2016;2016:9682757
pubmed: 26681958
Cell Stem Cell. 2018 Jun 01;22(6):824-833
pubmed: 29859173
Sci Rep. 2013;3:1658
pubmed: 23584574
Biomaterials. 2003 Jun;24(13):2309-16
pubmed: 12699668
World J Stem Cells. 2014 Nov 26;6(5):526-39
pubmed: 25426250
Transplantation. 2004 Feb 15;77(3):379-85
pubmed: 14966411
PLoS One. 2015 Oct 07;10(10):e0137494
pubmed: 26444683
Development. 2016 May 15;143(10):1674-87
pubmed: 26989177

Auteurs

Mitsuyoshi Nakao (M)

Cell Sheet Tissue Engineering Center (CSTEC), Department of Pharmaceutics and Pharmaceutical Chemistry, University of Utah, Health Sciences, 30 South 2000 East, Salt Lake City, UT, 84112, USA.
Faculty of Pharmacy, Keio University, 1-5-30 Shibakoen, Minato-ku, Tokyo, 105-8512, Japan.

Kyungsook Kim (K)

Cell Sheet Tissue Engineering Center (CSTEC), Department of Pharmaceutics and Pharmaceutical Chemistry, University of Utah, Health Sciences, 30 South 2000 East, Salt Lake City, UT, 84112, USA. kyungsook.kim@utah.edu.
ORCID: 0000-0002-0580-3510

Kenichi Nagase (K)

Faculty of Pharmacy, Keio University, 1-5-30 Shibakoen, Minato-ku, Tokyo, 105-8512, Japan.

David W Grainger (DW)

Cell Sheet Tissue Engineering Center (CSTEC), Department of Pharmaceutics and Pharmaceutical Chemistry, University of Utah, Health Sciences, 30 South 2000 East, Salt Lake City, UT, 84112, USA.

Hideko Kanazawa (H)

Faculty of Pharmacy, Keio University, 1-5-30 Shibakoen, Minato-ku, Tokyo, 105-8512, Japan.

Teruo Okano (T)

Cell Sheet Tissue Engineering Center (CSTEC), Department of Pharmaceutics and Pharmaceutical Chemistry, University of Utah, Health Sciences, 30 South 2000 East, Salt Lake City, UT, 84112, USA.
Institute of Advanced Biomedical Engineering and Science, Tokyo Women's Medical University, 8-1 Kawada-cho, Shinjuku-ku, Tokyo, 162-8666, Japan.

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Classifications MeSH

questionsmedicales.fr › Phénomènes physiologiques cellulaires › Adhérence cellulaire › Phenotypic traits of mesenchymal stem cell...
questionsmedicales.fr › Techniques d'investigation › Techniques in vitro › Techniques de culture › Techniques de culture cellulaire › Phenotypic traits of mesenchymal stem cell...
questionsmedicales.fr › Cellules › Structures cellulaires › Espace extracellulaire › Matrice extracellulaire › Phenotypic traits of mesenchymal stem cell...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Phenotypic traits of mesenchymal stem cell...
questionsmedicales.fr › Cellules › Structures cellulaires › Membrane cellulaire › Structures de la membrane cellulaire › Jonctions intercellulaires › Phenotypic traits of mesenchymal stem cell...
questionsmedicales.fr › Cellules › Cellules souches › Cellules souches multipotentes › Cellules souches mésenchymateuses › Phenotypic traits of mesenchymal stem cell...