Identification and characterization of α-xylosidase involved in xyloglucan degradation in Aspergillus oryzae.
Aspergillus oryzae
Glycoside hydrolase family 31
Xyloglucan
α-xylosidase
Journal
Applied microbiology and biotechnology
ISSN: 1432-0614
Titre abrégé: Appl Microbiol Biotechnol
Pays: Germany
ID NLM: 8406612
Informations de publication
Date de publication:
Jan 2020
Jan 2020
Historique:
received:
22
08
2019
accepted:
04
11
2019
revised:
21
10
2019
pubmed:
30
11
2019
medline:
2
7
2020
entrez:
30
11
2019
Statut:
ppublish
Résumé
Aspergillus oryzae produces hydrolases involved in xyloglucan degradation and induces the expression of genes encoding xyloglucan oligosaccharide hydrolases in the presence of xyloglucan oligosaccharides. A gene encoding α-xylosidase (termed AxyA), which is induced in the presence of xyloglucan oligosaccharides, is identified and expressed in Pichia pastoris. AxyA is a member of the glycoside hydrolase family 31 (GH31). AxyA hydrolyzes isoprimeverose (α-D-xylopyranosyl-(1→6)-D-glucopyranose) into D-xylose and D-glucose and shows hydrolytic activity with other xyloglucan oligosaccharides such as XXXG (heptasaccharide, Glc
Identifiants
pubmed: 31781819
doi: 10.1007/s00253-019-10244-z
pii: 10.1007/s00253-019-10244-z
doi:
Substances chimiques
Disaccharides
0
Glucans
0
Oligosaccharides
0
Xylans
0
xyloglucan
37294-28-3
isoprimeverose
534-98-5
Xylose
A1TA934AKO
Xylosidases
EC 3.2.1.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
201-210Subventions
Organisme : Japan Society for the Promotion of Science
ID : 18H02132