Metabolic requirements of Besnoitia besnoiti tachyzoite-triggered NETosis.
ATP
Besnoitia besnoiti
Glycolysis
Metabolic signatures
NETosis
PMN
Journal
Parasitology research
ISSN: 1432-1955
Titre abrégé: Parasitol Res
Pays: Germany
ID NLM: 8703571
Informations de publication
Date de publication:
Feb 2020
Feb 2020
Historique:
received:
12
04
2019
accepted:
06
11
2019
pubmed:
30
11
2019
medline:
10
4
2020
entrez:
30
11
2019
Statut:
ppublish
Résumé
Besnoitia besnoiti is the causative agent of bovine besnoitiosis, a disease affecting both, animal welfare and cattle productivity. NETosis represents an important and early host innate effector mechanism of polymorphonuclear neutrophils (PMN) that also acts against B. besnoiti tachyzoites. So far, no data are available on metabolic requirements of B. besnoiti tachyzoite-triggered NETosis. Therefore, here we analyzed metabolic signatures of tachyzoite-exposed PMN and determined the relevance of distinct PMN-derived metabolic pathways via pharmacological inhibition experiments. Overall, tachyzoite exposure induced a significant increase in glucose and serine consumption as well as glutamate production in PMN. Moreover, tachyzoite-induced cell-free NETs were significantly diminished via PMN pre-treatments with oxamate and dichloroacetate which both induce an inhibition of lactate release as well as oxythiamine, which inhibits pyruvate dehydrogenase, α-ketoglutarate dehydrogenase, and transketolase, thereby indicating a key role of pyruvate- and lactate-mediated metabolic pathways for proper tachyzoite-mediated NETosis. Furthermore, NETosis was increased by enhanced pH conditions; however, inhibitors of MCT-lactate transporters (AR-C141900, AR-C151858) failed to influence NET formation. Moreover, a significant reduction of tachyzoite-induced NET formation was also achieved by treatments with oligomycin A (inhibitor of ATP synthase) and NF449 (purinergic receptor P2X
Identifiants
pubmed: 31782011
doi: 10.1007/s00436-019-06543-z
pii: 10.1007/s00436-019-06543-z
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
545-557Références
Crit Care. 2004 Oct;8(5):331-6
pubmed: 15469594
Blood. 2011 Jan 20;117(3):953-9
pubmed: 20974672
Front Immunol. 2017 Feb 28;8:184
pubmed: 28293240
J Membr Biol. 1995 Jul;146(1):101-11
pubmed: 7563033
Front Immunol. 2018 Nov 12;9:2564
pubmed: 30483253
Nat Rev Immunol. 2006 Mar;6(3):173-82
pubmed: 16498448
Vet Parasitol. 2010 Nov 24;174(1-2):30-6
pubmed: 20850933
Parasitol Res. 2016 May;115(5):1991-2001
pubmed: 26847631
Vet Immunol Immunopathol. 1986 Sep;13(1-2):97-110
pubmed: 3765370
Immunology. 2015 Jun;145(2):213-24
pubmed: 25545227
Exp Hematol Oncol. 2013 Jul 27;2:18
pubmed: 23890079
Vet Immunol Immunopathol. 2010 Jan 15;133(1):1-8
pubmed: 19625090
Allergy Asthma Clin Immunol. 2006 Sep 15;2(3):98-108
pubmed: 20525154
Cell Mol Life Sci. 2007 Jun;64(12):1471-83
pubmed: 17375261
J Clin Invest. 1985 Sep;76(3):1079-89
pubmed: 2995444
Purinergic Signal. 2009 Mar;5(1):75-89
pubmed: 18600475
PLoS One. 2015 Sep 04;10(9):e0137221
pubmed: 26340269
Semin Immunopathol. 2013 Jul;35(4):439-53
pubmed: 23649713
J Cell Biol. 2010 Nov 1;191(3):677-91
pubmed: 20974816
J Biol Chem. 2014 Sep 26;289(39):26794-803
pubmed: 25104353
Cell Death Differ. 2004 Feb;11(2):143-53
pubmed: 14576767
Malar J. 2008 Feb 29;7:41
pubmed: 18312656
PLoS One. 2014 Nov 05;9(11):e111888
pubmed: 25372699
J Reticuloendothel Soc. 1980 Feb;27(2):189-200
pubmed: 7359510
Trends Immunol. 2009 Nov;30(11):547-56
pubmed: 19783480
J Immunol. 1999 Apr 15;162(8):4849-57
pubmed: 10202029
J Histochem Cytochem. 2004 Nov;52(11):1483-93
pubmed: 15505343
Proc Natl Acad Sci U S A. 2017 Apr 25;114(17):4483-4488
pubmed: 28396412
Mol Aspects Med. 2013 Apr-Jun;34(2-3):337-49
pubmed: 23506875
Pharmacol Rev. 1998 Sep;50(3):413-92
pubmed: 9755289
Immunol Lett. 2015 Oct;167(2):72-86
pubmed: 26209187
Blood. 1979 Jun;53(6):1133-9
pubmed: 36182
J Leukoc Biol. 2001 Apr;69(4):522-30
pubmed: 11310837
Front Immunol. 2013 Jan 21;3:424
pubmed: 23346086
Nat Commun. 2018 Jul 27;9(1):2958
pubmed: 30054480
Science. 2004 Mar 5;303(5663):1532-5
pubmed: 15001782
Biology (Basel). 2019 Mar 09;8(1):null
pubmed: 30857289
J Immunol. 2017 Jan 1;198(1):428-442
pubmed: 27903742
J Immunol. 1981 Feb;126(2):478-81
pubmed: 7451986
PLoS One. 2012;7(2):e31905
pubmed: 22384096
J Clin Invest. 1982 Sep;70(3):550-7
pubmed: 7107894
Vet Res Commun. 2008 Apr;32(4):325-32
pubmed: 18158611
Eur J Pharmacol. 2014 Dec 15;745:249-53
pubmed: 25445042
Proc Soc Exp Biol Med. 1971 Oct;138(1):350-2
pubmed: 5125534
Mediators Inflamm. 2016;2016:5898074
pubmed: 27445437
J Biomed Biotechnol. 2010;2010:427694
pubmed: 20454640
Front Immunol. 2018 Jan 09;8:1849
pubmed: 29375550
PLoS Biol. 2016 Mar 16;14(3):e1002404
pubmed: 26981861
Front Immunol. 2018 May 08;9:968
pubmed: 29867950
PLoS One. 2013 Nov 01;8(11):e78935
pubmed: 24223867
J Biol Chem. 1997 Feb 21;272(8):4941-52
pubmed: 9030554
J Periodontol. 1996 May;67(5):472-7
pubmed: 8724704
Science. 2006 Dec 15;314(5806):1792-5
pubmed: 17170310
Can J Microbiol. 2005 Oct;51(10):833-9
pubmed: 16333342
Curr Pharm Des. 2012;18(10):1319-30
pubmed: 22360558
IUBMB Life. 2012 Jan;64(1):1-9
pubmed: 22131303
Parasitol Res. 2016 May;115(5):2023-34
pubmed: 26852124
J Mol Cell Biol. 2010 Aug;2(4):176-7
pubmed: 20671113
J Immunol. 2003 Feb 15;170(4):1964-72
pubmed: 12574365
Front Immunol. 2019 May 22;10:1131
pubmed: 31191523
J Lab Clin Med. 1995 Mar;125(3):392-401
pubmed: 7897306
Parasitology. 2014 Sep;141(11):1406-17
pubmed: 24694568
Front Immunol. 2016 Dec 09;7:583
pubmed: 28018350
Biochim Biophys Acta. 2015 Feb;1853(2):299-307
pubmed: 25461841
PLoS One. 2013;8(1):e54205
pubmed: 23342104
Blood Adv. 2018 Aug 14;2(15):2029-2038
pubmed: 30108111
J Innate Immun. 2018;10(5-6):414-421
pubmed: 29909412
J Cell Biol. 2007 Jan 15;176(2):231-41
pubmed: 17210947
Methods Mol Biol. 2014;1124:307-18
pubmed: 24504961
Nat Commun. 2016 Feb 15;7:10555
pubmed: 26877061
PLoS One. 2014 Mar 11;9(3):e91415
pubmed: 24618849
J Immunol. 2007 Dec 15;179(12):8544-53
pubmed: 18056402
Front Immunol. 2017 May 29;8:606
pubmed: 28611772
J Biol Chem. 2018 Jun 22;293(25):9604-9613
pubmed: 29739849
Sci Signal. 2010 Jun 08;3(125):ra45
pubmed: 20530802