Interleukin 2 regulates the activation of human basophils.
Apoptosis
/ drug effects
Basophils
/ drug effects
Cells, Cultured
Dermatitis, Atopic
/ genetics
Eczema
/ genetics
Gene Expression Profiling
/ methods
Gene Expression Regulation
/ drug effects
Granulocyte-Macrophage Colony-Stimulating Factor
/ genetics
Humans
Interleukin-2
/ pharmacology
Interleukin-5
/ genetics
Pemphigoid, Bullous
/ genetics
Receptors, Interleukin-2
/ genetics
Skin
/ drug effects
Basophils
Interleukin 2
Interleukin 5
Skin diseases
Journal
Cytokine
ISSN: 1096-0023
Titre abrégé: Cytokine
Pays: England
ID NLM: 9005353
Informations de publication
Date de publication:
03 2020
03 2020
Historique:
received:
02
10
2019
revised:
12
11
2019
accepted:
15
11
2019
pubmed:
30
11
2019
medline:
24
8
2021
entrez:
30
11
2019
Statut:
ppublish
Résumé
Basophils are important effector cells in allergic disorders and anti-parasitic immune response. A number of activators including interleukin 3 (IL-3) and IgE have been identified in the regulation of human basophils expressing mediators such as histamine and leukotriene C4 (LTC4) and cytokines, including IL-4 and IL-13. Human basophils express high levels of IL-2 receptors. However, the function of the IL-2 pathway in basophils remains unknown. Here, we identified that IL-2 induced the activation of human basophils in vitro to express a variety of inflammatory cytokines and chemokines including IL-5, IL-13, GM-CSF and CCL-17. This effect by IL-2 is confirmed by an upstream regulator analysis using Ingenuity pathway analysis. Of note, one of the top regulated cytokines, IL-5, was for the first time identified to be induced by IL-2 in human basophils rather than IL-3 or anti-IgE. Immunofluorescence analysis of skin specimens from bullous pemphigoid and eczema revealed that infiltrating basophils in skin lesions widely expressed IL-5 and GM-CSF. Together, our findings reveal IL-2 as a novel regulator of human basophils. This adds a new layer to support the importance of basophils in allergic disorders.
Identifiants
pubmed: 31783259
pii: S1043-4666(19)30363-1
doi: 10.1016/j.cyto.2019.154934
pii:
doi:
Substances chimiques
Interleukin-2
0
Interleukin-5
0
Receptors, Interleukin-2
0
Granulocyte-Macrophage Colony-Stimulating Factor
83869-56-1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
154934Informations de copyright
Copyright © 2019 Elsevier Ltd. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.