Proteomic analysis of Nrk gene-disrupted placental tissue cells explains physiological significance of NRK.


Journal

BMC research notes
ISSN: 1756-0500
Titre abrégé: BMC Res Notes
Pays: England
ID NLM: 101462768

Informations de publication

Date de publication:
29 Nov 2019
Historique:
received: 05 10 2019
accepted: 21 11 2019
entrez: 1 12 2019
pubmed: 1 12 2019
medline: 18 4 2020
Statut: epublish

Résumé

NRK is a unique X chromosome-linked protein kinase expressed predominantly in placenta. The gene knockout causes placental overgrowth and delayed labor of Nrk-null fetuses from dams in mouse. To clarify unknown mechanisms behind the Nrk-null phenotypes, protein expression profiles were analyzed in the Nrk-null placenta using a high-performance two-dimensional electrophoresis methodology. Among around 1800 spots detected, we characterized a dozen protein spots whose expression levels were significantly altered in the Nrk-null placenta compared to wild-type. Analyzing these data sets is expected to reflect the difference physiologically in the presence or absence of NRK, facilitating the development of therapeutic strategies.

Identifiants

pubmed: 31783915
doi: 10.1186/s13104-019-4818-7
pii: 10.1186/s13104-019-4818-7
pmc: PMC6884884
doi:

Substances chimiques

Intracellular Signaling Peptides and Proteins 0
Nik related kinase EC 2.7.1.11
Protein Serine-Threonine Kinases EC 2.7.11.1

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

785

Subventions

Organisme : Grants-in-aid from the Ministry of Education, Culture, Sports, Science, and Technology of Japan
ID : 15K14378
Organisme : Science and Technology Innovative Research Team in Higher Educational Institutions of Hunan Province (CN)
ID : 21113505

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Auteurs

Kimitoshi Denda (K)

School of Life Science and Technology, Tokyo Institute of Technology, (M6-6) 2-12-1 Ookayama, Meguro-ku, Tokyo, 152-8550, Japan.

Kanako Ida (K)

School of Life Science and Technology, Tokyo Institute of Technology, (M6-6) 2-12-1 Ookayama, Meguro-ku, Tokyo, 152-8550, Japan.

Masataka Tanno (M)

Department of Pathology, Tokyo Nishi Tokushukai Hospital, Akishima, Japan.

Kanako Nakao-Wakabayashi (K)

School of Life Science and Technology, Tokyo Institute of Technology, (M6-6) 2-12-1 Ookayama, Meguro-ku, Tokyo, 152-8550, Japan.

Masayuki Komada (M)

Cell Biology Unit, Institute of Innovative Research, Tokyo Institute of Technology, Yokohama, Japan.

Nobuhiro Hayashi (N)

School of Life Science and Technology, Tokyo Institute of Technology, (M6-6) 2-12-1 Ookayama, Meguro-ku, Tokyo, 152-8550, Japan. nhayashi@bio.titech.ac.jp.

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Classifications MeSH