Agrimonia pilosa polysaccharide and its sulfate derives facilitate cell proliferation and osteogenic differentiation of MC3T3-E1 cells by targeting miR-107.
Agrimonia pilos polysaccharide
Steroid-induced avascular necrosis of the femoral head (SANFH)
miR-107
Journal
International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578
Informations de publication
Date de publication:
15 Aug 2020
15 Aug 2020
Historique:
received:
25
10
2019
revised:
16
11
2019
accepted:
26
11
2019
pubmed:
2
12
2019
medline:
19
3
2021
entrez:
2
12
2019
Statut:
ppublish
Résumé
The present study showed that low level of miR-107 in femoral head tissues from rats suffering steroid-induced osteonecrosis of the femoral head (SANFH) was associated with high degree of osteonecrosis and apoptosis. In vitro assay, pretreatment with an Agrimonia pilos polysaccharide (APP-AW) and its sulphated derivatives (S2) at 50 μg/ml for 48 h or overexpressing miR-107 were able to prevent cytotoxicity induced by 1 μM dexamethasone (DEX) in MC3T3-E1 cells via inhibition of apoptosis. Meanwhile, the decreased ALP activity, collagen content, BMP2, Runx2, OSX and OCN protein expression in DEX-treated MC3T3-E1 cells were rescued by the addition of APP-AW and S2, or miR-107 transfection. Moreover, DEX-induced increase of Bax, cytochrome c and caspase-3, as well as decrease of Bcl-2, Wnt3, β-catenin and c-Myc protein expression in MC3T3-E1 cells were also reversed. These findings suggest that APP-AW and S2 promote cell proliferation and osteogenic differentiation by enhancing miR-107 during the development of SANFH.
Identifiants
pubmed: 31786295
pii: S0141-8130(19)38686-6
doi: 10.1016/j.ijbiomac.2019.11.213
pii:
doi:
Substances chimiques
Biomarkers
0
MIRN107 microRNA, rat
0
MicroRNAs
0
Polysaccharides
0
Sulfates
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
616-625Informations de copyright
Copyright © 2019 Elsevier B.V. All rights reserved.